Telang N T
Prog Clin Biol Res. 1986;222:707-28.
The results obtained from the experiments described in this article may be summarized as follows: Mammary glands from adult mice of various strains can be maintained in a chemically defined medium for at least 23 days. The mammary epithelium in this in vitro system responds to selected steroid and polypeptide hormones in a manner similar to that observed in the explant cultures of immature mice. The incidence of spontaneous or carcinogen-induced lactogenic hormone-independent, putative preneoplastic MAL has a strong correlation with the age of mouse and the risk of developing mammary cancer. Selected fatty acids that are known to modulate mammary tumor incidence are also able to alter MAL incidence in mammary gland organ cultures from a high cancer incidence strain of mouse. Nontransformed mammary epithelial cells in culture respond to the potent mammary carcinogen DMBA by exhibiting increased proliferation and specific mutation that renders the cells resistant to cytotoxic TG. Selected fatty acids modulate DMBA-induced changes, at least in part, by modulating the process of metabolic cooperation between normal and mutant phenotypes. The present in vitro assays could be valuable to evaluate the direct effects of agents that initiate, promote and/or inhibit neoplastic transformation in mammary epithelium.
来自不同品系成年小鼠的乳腺可在化学成分明确的培养基中维持至少23天。该体外系统中的乳腺上皮对选定的类固醇和多肽激素的反应方式，与在未成熟小鼠的外植体培养中观察到的相似。自发或致癌物诱导的、不依赖催乳素的、假定的肿瘤前体MAL的发生率与小鼠年龄以及患乳腺癌的风险密切相关。已知可调节乳腺肿瘤发生率的特定脂肪酸，也能够改变高癌症发生率品系小鼠乳腺器官培养物中的MAL发生率。培养中的未转化乳腺上皮细胞对强效乳腺致癌物DMBA有反应，表现为增殖增加和特定突变，使细胞对细胞毒性TG产生抗性。特定脂肪酸至少部分地通过调节正常和突变表型之间的代谢合作过程，来调节DMBA诱导的变化。目前的体外检测方法对于评估引发、促进和/或抑制乳腺上皮肿瘤转化的药物的直接作用可能具有重要价值。
