Smartphone Nanocolorimetric Determination of Hydrogen Sulfide in Biosamples after Silver-Gold Core-Shell Nanoprism-Based Headspace Single-Drop Microextraction.

In this work, the sensitive detection of hydrogen sulfide (HS) was realized at low cost and high efficiency through the application of silver-gold core-shell nanoprism (Ag@Au-np) combined with headspace single-drop microextraction (HS-SDME). After SDME, smartphone nanocolorimetry (SNC), with the aid of a smartphone camera and color picker software, was used to detect and quantify the HS. The method took advantage of the inhibition of the ultraviolet-visible (UV-vis) signal caused by HS etching of the Ag@Au-np preadded to the SDME solvent to measure the HS concentration. The coating of the gold layer not only ensured the high stability of the nanomaterial but also enhanced the selectivity toward HS. The HS-SDME method was simple to process and required only a droplet of solvent for analysis to be realized. This HS-SDME-SCN approach exhibited a calibration graph linearity of between 0.1 and 100 μM and a limit of detection of 65 nM (relative standard deviations of N% ( n = 3) < 4.80). A comparison with UV-vis spectrophotometry was conducted. The practical applicability of HS-SDME-SNC was successfully demonstrated by determining HS in genuine biosamples (egg and milk).