School of Environmental and Chemical Engineering , Jiangsu University of Science and Technology , Zhenjiang 212003 , Jiangsu Province , PR China.
Department of Chemistry , National University of Singapore , 3 Science Drive 3 , Singapore 117543 , Singapore.
Anal Chem. 2019 May 7;91(9):5888-5895. doi: 10.1021/acs.analchem.9b00255. Epub 2019 Apr 24.
In this work, the sensitive detection of hydrogen sulfide (HS) was realized at low cost and high efficiency through the application of silver-gold core-shell nanoprism (Ag@Au-np) combined with headspace single-drop microextraction (HS-SDME). After SDME, smartphone nanocolorimetry (SNC), with the aid of a smartphone camera and color picker software, was used to detect and quantify the HS. The method took advantage of the inhibition of the ultraviolet-visible (UV-vis) signal caused by HS etching of the Ag@Au-np preadded to the SDME solvent to measure the HS concentration. The coating of the gold layer not only ensured the high stability of the nanomaterial but also enhanced the selectivity toward HS. The HS-SDME method was simple to process and required only a droplet of solvent for analysis to be realized. This HS-SDME-SCN approach exhibited a calibration graph linearity of between 0.1 and 100 μM and a limit of detection of 65 nM (relative standard deviations of N% ( n = 3) < 4.80). A comparison with UV-vis spectrophotometry was conducted. The practical applicability of HS-SDME-SNC was successfully demonstrated by determining HS in genuine biosamples (egg and milk).
在这项工作中，通过将银金核壳纳米棱镜（Ag@Au-np）与顶空单滴微萃取（HS-SDME）相结合，实现了低成本、高效率的硫化氢（HS）灵敏检测。在 SDME 之后，智能手机纳米比色法（SNC）利用智能手机摄像头和颜色选择器软件来检测和定量 HS。该方法利用 HS 对预先添加到 SDME 溶剂中的 Ag@Au-np 的紫外可见（UV-vis）信号的抑制作用来测量 HS 浓度。金层的涂层不仅确保了纳米材料的高稳定性，而且增强了对 HS 的选择性。HS-SDME 方法处理简单，仅需一滴溶剂即可实现分析。这种 HS-SDME-SCN 方法的校准曲线线性范围为 0.1 至 100 μM，检测限为 65 nM（相对标准偏差 N%（n = 3）<4.80）。与紫外可见分光光度法进行了比较。通过测定真实生物样品（鸡蛋和牛奶）中的 HS，成功证明了 HS-SDME-SNC 的实际适用性。
