Extracellular matrix components modulate different stages in β-microglobulin amyloid formation.

Amyloid deposition of WT human β-microglobulin (WT-hβm) in the joints of long-term hemodialysis patients is the hallmark of dialysis-related amyloidosis. , WT-hβm does not form amyloid fibrils at physiological pH and temperature unless co-solvents or other reagents are added. Therefore, understanding how fibril formation is initiated and maintained in the joint space is important for elucidating WT-hβm aggregation and dialysis-related amyloidosis onset. Here, we investigated the roles of collagen I and the commonly administered anticoagulant, low-molecular-weight (LMW) heparin, in the initiation and subsequent aggregation phases of WT-hβm in physiologically relevant conditions. Using thioflavin T fluorescence to study the kinetics of amyloid formation, we analyzed how these two agents affect specific stages of WT-hβm assembly. Our results revealed that LMW-heparin strongly promotes WT-hβm fibrillogenesis during all stages of aggregation. However, collagen I affected WT-hβm amyloid formation in contrasting ways: decreasing the lag time of fibril formation in the presence of LMW-heparin and slowing the rate at higher concentrations. We found that in self-seeded reactions, interaction of collagen I with WT-hβm amyloid fibrils attenuates surface-mediated growth of WT-hβm fibrils, demonstrating a key role of secondary nucleation in WT-hβm amyloid formation. Interestingly, collagen I fibrils did not suppress surface-mediated assembly of WT-hβm monomers when cross-seeded with fibrils formed from the N-terminally truncated variant ΔN6-hβm. Together, these results provide detailed insights into how collagen I and LMW-heparin impact different stages in the aggregation of WT-hβm into amyloid, which lead to dramatic effects on the time course of assembly.