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棕榈心蛋白质溶解度的动力学研究以及棕榈心分离蛋白的物理化学、功能和热性质研究。

Kinetic studies on palm heart protein solubility and investigation of physicochemical, functional and thermal properties of palm heart protein isolate.

作者信息

Hematian Sourki Abdollah, Rahmanian Mahsa

机构信息

1Department of Food Science and Technology, Faculty of Agriculture, Jahrom University, PO Box: 74135-111, Jahrom, Iran.

Kherad Institute of Higher Education, Bushehr, Iran.

出版信息

J Food Sci Technol. 2019 Apr;56(4):1820-1828. doi: 10.1007/s13197-019-03626-8. Epub 2019 Feb 18.

Abstract

The chemical composition of palm heart (cultivar Piarom) and solubility kinetics of its protein was investigated under different salt concentrations and pHs. Palm heart protein isolate (PHPI) was prepared using alkaline extraction and acidic precipitation and its functional properties (emulsifying properties and emulsion stability, foam capacity, and water holding and fat absorption capacity) and thermal properties were determined. It was revealed that palm heart had high nutritional value and contains high protein content. By increasing pH up to 3, solubility was decreased and then significantly increased by elevating pH to 9 ( < 0.05). Salt addition significantly decreased palm heart protein (PHP) solubility. Considering solubility kinetics, pH  9 and salt concentration of 0.25 M was determined as the best protein extraction condition. Denaturation temperature and enthalpy changes of PHPI were 108.63 °C and 143.1 J/g; suggesting high thermal stability of this product. Regarding its suitable functional, physicochemical and thermal properties, PHP can be considered as an ideal substitution for animal protein sources in the food industry.

摘要

研究了不同盐浓度和pH值下棕榈心(品种Piarom)的化学成分及其蛋白质的溶解动力学。采用碱提取和酸沉淀法制备了棕榈心分离蛋白(PHPI),并测定了其功能特性(乳化特性和乳液稳定性、泡沫容量以及持水和吸油能力)和热特性。结果表明,棕榈心具有较高的营养价值且蛋白质含量高。将pH值提高到3时,溶解度降低,然后将pH值提高到9时溶解度显著增加(<0.05)。添加盐显著降低了棕榈心蛋白(PHP)的溶解度。考虑到溶解动力学,确定pH值为9且盐浓度为0.25 M为最佳蛋白质提取条件。PHPI的变性温度和焓变为108.63℃和143.1 J/g;表明该产品具有较高的热稳定性。鉴于其合适的功能、物理化学和热特性,PHP可被视为食品工业中动物蛋白来源的理想替代品。

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