Seow Eng-Keng, Gan Chee-Yuen, Tan Thuan-Chew, Lee Lai Kuan, Easa Azhar Mat
1Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia (USM), 11800 Penang, Malaysia.
2Analytical Biochemistry Research Center, Universiti Sains Malaysia (USM), 11800 Penang, Malaysia.
J Food Sci Technol. 2019 Apr;56(4):2105-2114. doi: 10.1007/s13197-019-03691-z. Epub 2019 Mar 6.
Present study compared the rheological properties of glutinous rice flour (GRF) gel (33.3%, w/v) added with raw bee honey (RBH) or stingless bee honey (SBH) with/without heating treatment. RBH (diatase activity: 12.14 Schade) and SBH (1.53 Schade) significantly reduced the network of GRF gel by lowering the gel viscosity, with RBH having the highest rate of viscosity decrease (- 2.74 × 10 Pa). As the addition of heated-SBH or heated-RBH did not reduce gel viscosity, it was hypothesised that active diastase played a major role to weaken gel network. This was further supported by the significant and the lowest storage modulus (G') value of RBH-GRF gel (5.99 ± 0.02 Pa), as compared to SBH-GRF (6.27 ± 0.04 Pa) and control (6.33 ± 0.04 Pa). A detail of rheological behaviour of the gels was further explained using power law. Overall, this GRF gel model has successfully demonstrated the potential of honey diastase in weakening network of starch-based food.
本研究比较了添加生蜂蜜(RBH)或无刺蜂蜂蜜(SBH)的糯米粉(GRF)凝胶(33.3%,w/v)在有无加热处理情况下的流变学特性。RBH(淀粉酶活性:12.14 Schade)和SBH(1.53 Schade)通过降低凝胶粘度显著减少了GRF凝胶网络,其中RBH的粘度降低速率最高(-2.74×10 Pa)。由于添加加热后的SBH或加热后的RBH并未降低凝胶粘度,因此推测活性淀粉酶在削弱凝胶网络中起主要作用。与SBH-GRF凝胶(6.27±0.04 Pa)和对照(6.33±0.04 Pa)相比,RBH-GRF凝胶的储能模量(G')值显著且最低(5.99±0.02 Pa),这进一步支持了上述推测。使用幂律进一步解释了凝胶的流变行为细节。总体而言,该GRF凝胶模型成功证明了蜂蜜淀粉酶在削弱淀粉基食品网络方面的潜力。