[急性白血病患者T淋巴细胞亚群及B淋巴细胞的检测与分析]
[Detection and Analysis of T Lymphocyte Subsets and B Lymphocytes in Patients with Acute Leukemia].
作者信息
Cheng Juan, Ma Hai-Zhen, Zhang Hao
机构信息
Department of Hematology, The First Hospital of Lanzhou University, Laznhou 730000, Gansu Province, China,E-mail:
Department of Hematology, The First Hospital of Lanzhou University, Laznhou 730000, Gansu Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Apr;27(2):327-330. doi: 10.19746/j.cnki.issn.1009-2137.2019.02.003.
OBJECTIVE
To investigate the changes of T lymphocyte subsets, B lymphocyte and NK cells in peripheral blood of patients with acute leukemia at different periods and their significance.
METHODS
The peripheral T lymphocyte subsets and B lymphocyte of 95 patients with acute leukemia [(43 cases of acute lymphoblastic leukemia (ALL), 52 cases of acute myeloid leukemia (AML)] and 50 normal people were detected by flow cytometry respectively.
RESULTS
The positive rate of CD3, CD3CD4, CD3CD8, NK cells and CD4/CD8 in the patients with newly diagnosed acute leukemia were significantly lower than those in normal controls (P<0.05) , but increased obviously after complete remission. The positive rate of Treg cells in the patients with newly diagnosed leukemia group was significantly higher than that in normal controls (P<0.01) , but decreased obviously after complete remission. Positive rate of CD3CD19 cells in the patients with newly diagnosed acute myeloid leukemia was significantly lower , but higher in the patients with newly diagnosed acute lymphoblastic leukemia than that in normal controls with statistical significant difference (P<0.05) , and increased obviously in the patients with acute myeloid leukemia (P<0.05) after complete remission , but decreased in the patients with acute lymphoblastic leukemia (P<0.01) after complete remission or no-remission.
CONCLUSION
Changes of T lymphocyte subsets, B lymphocytes and NK cells in the patients with newly diagnosed acute leukemia are significant, thus the detection of T lymphocyte cell subsets, B lymphocytes and NK cells can provide some evidences. for evaluation of the disease severity, curative efficiency and prognosis of patients with acute leukemia.
目的
探讨急性白血病患者不同时期外周血T淋巴细胞亚群、B淋巴细胞及NK细胞的变化及其意义。
方法
采用流式细胞术分别检测95例急性白血病患者（其中急性淋巴细胞白血病43例、急性髓系白血病52例）及50例正常人外周血T淋巴细胞亚群和B淋巴细胞。
结果
初诊急性白血病患者CD3、CD3CD4、CD3CD8、NK细胞阳性率及CD4/CD8比值均显著低于正常对照组（P<0.05），完全缓解后明显升高。初诊白血病组调节性T细胞阳性率显著高于正常对照组（P<0.01），完全缓解后明显降低。初诊急性髓系白血病患者CD3CD19细胞阳性率显著低于正常对照组，初诊急性淋巴细胞白血病患者高于正常对照组，差异有统计学意义（P<0.05）；急性髓系白血病患者完全缓解后明显升高（P<0.05），急性淋巴细胞白血病患者完全缓解或未缓解后均降低（P<0.01）。
结论
初诊急性白血病患者T淋巴细胞亚群、B淋巴细胞及NK细胞变化显著，检测T淋巴细胞亚群、B淋巴细胞及NK细胞可为评估急性白血病患者病情严重程度、疗效及预后提供一定依据。
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