Guimarães Fabio Tadeu Lourenço, Ferreira Rodrigo Novaes, Brito-Melo Gustavo Eustáquio Alvim, Rocha-Vieira Etel, Pereira Wagner de Fátima, Pinheiro Sérgio Veloso Brant, Miranda Aline Silva, Simões E Silva Ana Cristina
Centro Integrado de Pós-Graduação e Pesquisa em Saúde - CIPq, Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM), Diamantina, Brazil.
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Front Pediatr. 2019 Apr 2;7:114. doi: 10.3389/fped.2019.00114. eCollection 2019.
Idiopathic nephrotic syndrome (INS) is classified according to the response to drug therapy in steroid-sensitive (SS), steroid-dependent (SD), and steroid-resistant (SR) categories. Previous studies showed changes in inflammatory activity of subpopulations of lymphocytes in INS. This study aimed to compare SS and SR patients in regard to subpopulations of leukocytes, profile of regulatory lymphocytes, and migratory activity of lymphocyte subpopulations. Results obtained in INS patients were also compared to age and sex-matched healthy controls. This is a cross-sectional study including SS patients ( = 30), SR patients ( = 14), and controls ( = 10). Peripheral blood samples were withdrawn for leukocyte flow cytometry analysis. Percentage of B-lymphocytes and natural killer (NK) cells were significantly reduced in SR patients when compared to controls, while the percentage of NKT cells were decreased in SS patients in comparison to controls. Percentages of CD4 expressing FoxP3 and CTLA4 were significantly higher in SS patients in comparison to SR patients and controls. The expression of integrin CD18 on the surface of T lymphocytes (CD3) was reduced in SS patients if compared to controls. This study found that SS INS patients have higher levels of regulatory T-lymphocytes and lower expression of adhesion molecules than SR patients.
特发性肾病综合征（INS）根据对药物治疗的反应分为激素敏感型（SS）、激素依赖型（SD）和激素抵抗型（SR）。先前的研究表明INS患者淋巴细胞亚群的炎症活性存在变化。本研究旨在比较SS和SR患者的白细胞亚群、调节性淋巴细胞谱以及淋巴细胞亚群的迁移活性。还将INS患者的结果与年龄和性别匹配的健康对照进行了比较。这是一项横断面研究，包括SS患者（n = 30）、SR患者（n = 14）和对照（n = 10）。采集外周血样本进行白细胞流式细胞术分析。与对照相比，SR患者的B淋巴细胞和自然杀伤（NK）细胞百分比显著降低，而与对照相比，SS患者的NKT细胞百分比降低。与SR患者和对照相比，SS患者中表达FoxP3和CTLA4的CD4百分比显著更高。与对照相比，SS患者T淋巴细胞（CD3）表面整合素CD18的表达降低。本研究发现，与SR患者相比，SS INS患者的调节性T淋巴细胞水平更高，粘附分子表达更低。
