Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, 30-387, Kraków, Poland.
Department of Zoology, Faculty of Biology and Environmental Protection, University of Silesia, Bankowa 9, 40-007, Katowice, Poland.
Protoplasma. 2019 Sep;256(5):1205-1215. doi: 10.1007/s00709-019-01377-z. Epub 2019 Apr 17.
The biological nature, ultrastructure, distribution, and mode of transmission between generations of the microorganisms associated with three species (Orthezia urticae, Matsucoccus pini, Steingelia gorodetskia) of primitive families (archaeococcoids = Orthezioidea) of scale insects were investigated by means of microscopic and molecular methods. In all the specimens of Orthezia urticae and Matsucoccus pini examined, bacteria Wolbachia were identified. In some examined specimens of O. urticae, apart from Wolbachia, bacteria Sodalis were detected. In Steingelia gorodetskia, the bacteria of the genus Sphingomonas were found. In contrast to most plant sap-sucking hemipterans, the bacterial associates of O. urticae, M. pini, and S. gorodetskia are not harbored in specialized bacteriocytes, but are dispersed in the cells of different organs. Ultrastructural observations have shown that bacteria Wolbachia in O. urticae and M. pini, Sodalis in O. urticae, and Sphingomonas in S. gorodetskia are transovarially transmitted from mother to progeny.
采用微观和分子方法，研究了与三种原始科（古球蚧科= Orthezioidea）的介壳虫（Orthezia urticae、Matsucoccus pini、Steingelia gorodetskia）相关的微生物的生物学特性、超微结构、分布和世代间传播方式。在所研究的 Orthezia urticae 和 Matsucoccus pini 的所有标本中，均鉴定出细菌 Wolbachia。在一些研究的 Orthezia urticae 标本中，除了 Wolbachia 之外，还检测到了 Sodalis 细菌。在 Steingelia gorodetskia 中，发现了属 Sphingomonas 的细菌。与大多数吸食植物汁液的半翅目昆虫不同，O. urticae、M. pini 和 S. gorodetskia 的细菌伴生物不是存在于专门的细菌细胞中，而是分散在不同器官的细胞中。超微结构观察表明，O. urticae 和 M. pini 中的细菌 Wolbachia、O. urticae 中的 Sodalis 和 S. gorodetskia 中的 Sphingomonas 是通过母代到后代的转卵传递的。
