狼疮肾炎患者 TLR9 和 IL-23 表达的相关性分析。
Correlation analysis of TLR9 and IL-23 expression in patients with lupus nephritis.
机构信息
Department of Rheumatology and Immunology, Affiliated Hospital of Jining Medical College, Jining, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):2944-2949. doi: 10.26355/eurrev_201904_17574.
OBJECTIVE
The aim of this study was to investigate the expression levels of toll-like receptor 9 (TLR9) and interleukin-23 (IL-23) in renal tissue and serum of patients with lupus nephritis (LN), and to explore their clinical correlation.
PATIENTS AND METHODS
LN patients and healthy controls were enrolled in the experimental group and the control group, respectively. Blood samples, serum, and peripheral blood mononuclear cells (PBMCs) were collected. Renal lesion tissues and adjacent normal tissues of LN patients were harvested from a renal tissue biopsy. Serum level of IL-23 was detected by enzyme-linked immunosorbent assay (ELISA). Expression of IL-23 in PBMCs was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Meanwhile, TLR9 expression in renal tissues was accessed by immunohistochemistry staining. Subsequently, 24-h protein urine, renal tubular pathological activity index, erythrocyte sedimentation rate (ESR), serum complement C3 level, and blood albumin level of LN patients were recorded. Also, the correlation between TLR9 expression and these pathological indexes was measured by correlation analysis.
RESULTS
Serum level of IL-23 in LN patients was significantly higher than that of healthy controls. Similarly, the mRNA and protein expressions of IL-23 in PBMCs of LN patients were markedly higher than those of healthy controls. IL-23 expression was positively correlated with renal tubular pathological activity index of LN patients. Meanwhile, TLR9 was highly expressed in renal tissues of LN patients. Furthermore, TLR9 expression was positively correlated with 24-h protein urine, renal tubular pathological activity index and ESR, whereas negatively correlated with serum complement C3 level and blood albumin level of LN patients.
CONCLUSIONS
IL-23 is highly expressed in the serum of LN patients, and its expression is closely related to the occurrence of LN. Also, the expression of TLR9 is up-regulated in the tubulointerstitium of LN patients, which is correlated with relevant clinical indexes.
目的
本研究旨在探讨狼疮肾炎（LN）患者肾组织和血清中 Toll 样受体 9（TLR9）和白细胞介素-23（IL-23）的表达水平，并探讨其临床相关性。
方法
分别将 LN 患者和健康对照者纳入实验组和对照组，采集血样、血清和外周血单个核细胞（PBMC）。采集 LN 患者肾组织活检的肾病变组织及相邻正常组织。采用酶联免疫吸附试验（ELISA）检测血清 IL-23 水平。通过实时定量聚合酶链反应（qRT-PCR）和 Western blot 分别检测 PBMCs 中 IL-23 的表达。同时，采用免疫组织化学染色法检测肾组织中 TLR9 的表达。随后，记录 LN 患者 24 小时尿蛋白、肾小管病理活动指数、红细胞沉降率（ESR）、血清补体 C3 水平和血白蛋白水平，并通过相关性分析测量 TLR9 表达与这些病理指标的相关性。
结果
LN 患者血清 IL-23 水平明显高于健康对照组。同样，LN 患者 PBMCs 中 IL-23 的 mRNA 和蛋白表达也明显高于健康对照组。IL-23 表达与 LN 患者肾小管病理活动指数呈正相关。同时，TLR9 在 LN 患者的肾组织中高表达。此外，TLR9 表达与 LN 患者的 24 小时尿蛋白、肾小管病理活动指数和 ESR 呈正相关，与血清补体 C3 水平和血白蛋白水平呈负相关。
结论
IL-23 在 LN 患者血清中高表达，其表达与 LN 的发生密切相关。此外，TLR9 在 LN 患者的肾小管间质中表达上调，与相关临床指标相关。
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