College of Resources and Environment, Southwest University, Chongqing, 400715, China.
Chongqing Engineering Research Center for Agricultural Non-point Source Pollution Control in the Three Gorges Reservoir Area, Chongqing, 400715, China.
Curr Microbiol. 2020 Apr;77(4):522-527. doi: 10.1007/s00284-019-01670-3. Epub 2019 Apr 19.
Mercury (Hg) methylation is mainly a microbial process mediated by anaerobes. The continued study of Pseudomonas putida (P. putida) strain TGRB4 genome was inspired by the fact that it can transform Hg into the highly toxic methylmercury (MeHg) under aerobic conditions. P. putida strain TGRB4 is a Gram-negative rod-shaped Gamma-proteobacterium (γ-proteobacterium), isolated from the soil in a typical water level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR), which suffered from seasonally water level alternations every year. Draft genome assembly of P. putida strain TGRB4 is presented here, which was sequenced using Illumina Hiseq and PacBio single-molecule real-time (SMRT) platforms. Its genome harbors a total of 5504 genes and a G + C content of 62.6%. We further identified the enzymes related to Hg methylation, and found two well-known methyltransferase, including 5-methyl-tetrahydrofolate (5-methyl-THF) and S-adenosylmethionine (AdoMet), were annotated in the genome of P. putida strain TGRB4. This genome information could be treated as a research material to further study the Hg methylation mechanisms under aerobic environment.
汞（Hg）的甲基化主要是一种由厌氧菌介导的微生物过程。对 Pseudomonas putida（P. putida）菌株 TGRB4 基因组的持续研究受到以下事实的启发：它可以在有氧条件下将汞转化为剧毒的甲基汞（MeHg）。P. putida 菌株 TGRB4 是一种革兰氏阴性杆状γ-变形菌（γ-proteobacterium），从三峡水库（TGR）典型的水位波动区（WLFZ）的土壤中分离出来，每年都会受到季节性水位变化的影响。这里呈现了对 P. putida 菌株 TGRB4 的基因组草图组装，它是使用 Illumina Hiseq 和 PacBio 单分子实时（SMRT）平台测序的。其基因组共包含 5504 个基因，G+C 含量为 62.6%。我们进一步鉴定了与 Hg 甲基化相关的酶，发现了两种著名的甲基转移酶，包括 5-甲基四氢叶酸（5-methyl-THF）和 S-腺苷甲硫氨酸（AdoMet），在 P. putida 菌株 TGRB4 的基因组中被注释。该基因组信息可作为研究材料，进一步研究有氧环境下 Hg 甲基化机制。
