State Key Laboratory of Hybrid Rice, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.
Rice Research Institute, Southwest University of Science and Technology, Mianyang, 621010, Sichuan, China.
Plant J. 2019 Aug;99(3):556-570. doi: 10.1111/tpj.14344. Epub 2019 May 14.
Meiosis is critical for sexual reproduction and the generation of new allelic variations in most eukaryotes. In this study, we report the isolation of a meiotic gene, DLC1, using a map-based cloning strategy. The dlc1 mutant is sterile in both male and female gametophytes due to an earlier defect in the leptotene chromosome and subsequent abnormalities at later stages. DLC1 is strongly expressed in the pollen mother cells (PMCs) and tapetum and encodes a nucleus-located rice type-B response regulator (RR) with transcriptional activity. Further investigations showed that DLC1 interacts with all five putative rice histidine phosphotransfer proteins (HPs) in yeast and planta cells, suggesting a possible participation of the two-component signalling systems (TCS) in rice meiosis. Our results demonstrated that DLC1 is required for rice meiosis and fertility, providing useful information for the role of TCS in rice meiosis.
减数分裂对于有性繁殖和大多数真核生物新等位基因变异的产生至关重要。在这项研究中，我们使用基于图谱的克隆策略报告了一个减数分裂基因 DLC1 的分离。由于细线期染色体的早期缺陷和随后的晚期异常，dlc1 突变体在雄性和雌性配子体中都是不育的。DLC1 在花粉母细胞（PMCs）和绒毡层中强烈表达，编码具有转录活性的细胞核定位的水稻 B 型反应调节剂（RR）。进一步的研究表明，DLC1 在酵母和植物细胞中与所有五个假定的水稻组氨酸磷酸转移蛋白（HP）相互作用，这表明双组分信号系统（TCS）可能参与了水稻减数分裂。我们的结果表明，DLC1 是水稻减数分裂和育性所必需的，为 TCS 在水稻减数分裂中的作用提供了有用的信息。
