Morohaku Kanako
School of Science and Technology, Institute of Agriculture, Division of Animal Science, Shinshu University, Nagano 399-4598, Japan.
Institute for Biomedical Sciences, Shinshu University, Nagano 399-4598, Japan.
J Reprod Dev. 2019 Aug 9;65(4):281-287. doi: 10.1262/jrd.2019-024. Epub 2019 Apr 20.
Eggs are female germ cells that are required for producing offspring through sexual reproduction. In mammals, eggs are produced in the ovary and ovulated into the oviduct. It is well known that over 99% of eggs are degenerated without ovulation, so that many studies have attempted in vitro folliculogenesis to produce many eggs in different species for a few decades. Although many methods have been developed, a success of in vitro egg production with the resultant live birth of offspring has been limited, especially in livestock animals. More recently, we have succeeded in producing live pups derived from in vitro/ex vivo egg production in mice. This review aims to introduce our recent findings with a brief history of in vitro/ex vivo culture systems for follicles and ovaries.
卵子是通过有性生殖产生后代所必需的雌性生殖细胞。在哺乳动物中，卵子在卵巢中产生并排卵进入输卵管。众所周知，超过99%的卵子在未排卵的情况下会退化，因此几十年来，许多研究都尝试通过体外卵泡发生在不同物种中产生大量卵子。尽管已经开发了许多方法，但通过体外卵子生产并最终实现后代活体出生的成功率一直有限，尤其是在家畜中。最近，我们成功地从小鼠的体外/体内卵子生产中培育出了活体幼崽。这篇综述旨在介绍我们最近的发现，并简要回顾卵泡和卵巢的体外/体内培养系统的历史。
