Bakhtiari Somaye, Hasanvand Banafsheh, Pajavand Hamid, Alvandi Amirhooshang, Abiri Ramin
Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
APMIS. 2019 Jul;127(7):510-514. doi: 10.1111/apm.12953. Epub 2019 May 29.
Loop-mediated isothermal amplification (LAMP) is a promising nucleic acid-based assay for quick, accurate and cost-effective diagnosis of many infectious agents. The purpose of this study was to assess the diagnostic value of LAMP for rapid and accurate detection of Helicobacter pylori in biopsy specimens. Patients suffering from one or several gastroduodenal disorders were enrolled in the study. Specificity, sensitivity, and the positive and negative predictive values of LAMP were compared with the gold standard result, which was the assembled result of culture, rapid urease test and polymerase chain reaction. Sensitivity, specificity, and the positive and negative predictive values of LAMP in comparison with the gold standard result were 100%, 30.76%, and 87.67% and 100% respectively [%95 CI]. As the diagnostic value of LAMP is favourable, the method is an optimum technique for diagnosis the presence of H. pylori in different clinical and environmental samples.
环介导等温扩增技术（LAMP）是一种很有前景的基于核酸的检测方法，可用于快速、准确且经济高效地诊断多种感染因子。本研究的目的是评估LAMP在活检标本中快速准确检测幽门螺杆菌的诊断价值。患有一种或多种胃十二指肠疾病的患者被纳入本研究。将LAMP的特异性、敏感性以及阳性和阴性预测值与金标准结果进行比较，金标准结果是培养、快速尿素酶试验和聚合酶链反应的综合结果。与金标准结果相比，LAMP的敏感性、特异性以及阳性和阴性预测值分别为100%、30.76%、87.67%和100%[95%CI]。由于LAMP的诊断价值良好，该方法是诊断不同临床和环境样本中幽门螺杆菌存在情况的最佳技术。
