Odontoblastic/osteogenic differentiation of human dental pulp stem cells (hDPSCs) is a key factor in tooth and pulp regeneration, but its mechanism still remains unknown. The purpose of this research is to look into the mechanism by which Stathmin affects the proliferation and odontoblastic/osteogenic differentiation of hDPSCs, and whether the Wnt/β- catenin is related to this regulation. First, the Stathmin expression was inhibited by lentiviral vector, after that the transcriptome sequencing technology was used to screen the differentially expressed genes, then we found Wnt5a which related to the regulation of Wnt/β-catenin was regulated. Comparing with hDPSC in the control group, the shRNA-Stathmin group inhibited proliferation and odontoblastic/osteogenic differentiation. The result of molecular analysis indicated that the Wnt/β-catenin was inhibited when Stathmin was silenced. After that, the shRNA-Stathmin group were added with LiCl (activator of Wnt/β-catenin), and the Wnt/β-catenin was significantly activated in β-catenin. After activation of the Wnt/β-catenin, the proliferation of hDPSCs was significantly increased and the expression of genes related to odontoblastic/osteogenic differentiation was also significantly increased. Taken together, these findings reveal for the first time that the Stathmin-Wnt/β-catenin plays a positive regulatory role in hDPSC proliferation and odontoblastic/osteogenic differentiation. SIGNIFICANCE: Transcriptome sequencing revealed that Stathmin interacts with Wnt/β-catenin signaling pathway-related proteins such as Wnt5a. At the same time, experiments have confirmed that Stathmin protein can affect the proliferation and odontogenetic differentiation of hDPSCs.The innovation of this paper is to link the Stathmin and Wnt/β-catenin signaling pathways for the first time, to explore the interaction of Stathmin and Wnt/β-catenin signaling pathways and the mechanism of this regulation on human dental pulp stem cells (hDPSCs) of odontoblastic/osteogenic differentiation and proliferation function. Especially for the regulation of odontoblastic/osteogenic differentiation, we have verified this mechanism at the molecular level and characterization leveland this regulation also provides new ideas for dental pulp tissue engineering. At the same time, more than 3000 proteins related to the change of Stathmin level were screened by transcriptome sequencing technology, which provided a possibility to further exploration of the regulation mechanism of Stathmin on various aspects of cell biological characteristics.