College of Science, Hebei Agricultural University, Baoding 071001, Hebei, China.
College of Science, Hebei Agricultural University, Baoding 071001, Hebei, China.
J Chromatogr A. 2019 Aug 30;1600:9-16. doi: 10.1016/j.chroma.2019.04.031. Epub 2019 Apr 13.
A solid phase extraction (SPE) method with a porous organic polymer as adsorbent was established for the extraction of five carbamate pesticides (metolcarb, carbaryl, isoprocarb, bassa and diethofencarb) from milk, white wine and juice samples prior to high performance liquid chromatography-diode array detection. The main factors affecting the SPE were optimized, including sample volume, sample loading rate, sample solution pH, desorption conditions and cartridge reusability. Under the optimal conditions, a good linearity existed in the range from 1.0 to 320.0 ng mL for milk and white wine samples and from 0.5 to 160.0 ng mL for juice sample with the correlation coefficients between 0.9956 and 0.9998. The method recoveries of the carbamates were 82.0%-110.0%. The limits of detection were 0.12-0.40 ng mL for milk and white wine samples, and 0.06-0.20 ng mL for juice sample. The adsorption mechanism of the adsorbent was investigated by the extraction of different types of organic compounds (carbamates, benzoylurea insecticides, endocrine disrupting chemicals and polycyclic aromatic hydrocarbons) with the adsorbent. The results showed that the existence of the hydrogen-bonding between the adsorbent and the analytes is favorable for the adsorption and strong hydrophobicity seems to have an adverse effect.
建立了一种以多孔有机聚合物为吸附剂的固相萃取（SPE）方法，用于从牛奶、白葡萄酒和果汁样品中提取五种氨基甲酸酯类农药（灭多威、西维因、异丙威、巴沙和苯氧威），然后进行高效液相色谱-二极管阵列检测。优化了影响 SPE 的主要因素，包括样品体积、样品加载速率、样品溶液 pH 值、洗脱条件和萃取柱的可重复使用性。在最佳条件下，牛奶和白葡萄酒样品的线性范围为 1.0-320.0ng mL，果汁样品的线性范围为 0.5-160.0ng mL，相关系数在 0.9956 到 0.9998 之间。氨基甲酸酯类农药的回收率为 82.0%-110.0%。牛奶和白葡萄酒样品的检测限为 0.12-0.40ng mL，果汁样品的检测限为 0.06-0.20ng mL。通过用该吸附剂萃取不同类型的有机化合物（氨基甲酸酯类、苯甲酰脲类杀虫剂、内分泌干扰化学物质和多环芳烃），研究了吸附剂的吸附机理。结果表明，吸附剂与分析物之间氢键的存在有利于吸附，而强疏水性似乎有不利影响。
