Oliveira Bruno César Miranda, Nagata Walter Bertequini, Arana David Giraldo, Ferreira Gabriela Cortellini, Sitton Heliton Aparecido, de Oliveira Marcelo Rodrigues Freitas, Meireles Marcelo Vasconcelos
Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, R. Clóvis Pestana 793, Jardim Dona Amélia, Araçatuba, São Paulo 16050-680, Brazil.
Secretaria Municipal de Meio Ambiente e Sustentabilidade de Araçatuba, Avenida Alcides Fagundes Chagas, 222, Araçatuba, São Paulo, Brazil.
Vet Parasitol Reg Stud Reports. 2017 Dec;10:154-156. doi: 10.1016/j.vprsr.2017.10.007. Epub 2017 Nov 1.
Cryptosporidiosis in birds manifests as an acute or chronic disease of the respiratory or digestive tracts. The objective of our study was to perform the molecular characterization of Cryptosporidium spp. in wild psittacines kept in captivity at the Araçatuba Municipal Zoo, São Paulo, Brazil. A total of 47 fecal samples were collected from Amazona aestiva, Psittacara leucophthalma, and Ara ararauna. All samples were collected at the time the birds defecated. DNA extraction was performed using the ZR Fecal DNA MiniPrep™ kit (Zymo Research). Screening for Cryptosporidium spp. was accomplished using nested PCR targeting the 18S RNA gene and sequencing of amplified fragments. Positivity for Cryptosporidium spp. (10.64%; 5/47) was found in A. ararauna (4) and P. leucophthalma (1) samples. The amplified fragments were sequenced and showed 100% genetic similarity with Cryptosporidium baileyi.
鸟类隐孢子虫病表现为呼吸道或消化道的急性或慢性疾病。我们研究的目的是对巴西圣保罗州阿拉萨图巴市立动物园圈养的野生鹦鹉体内的隐孢子虫进行分子特征分析。总共从绿颊亚马逊鹦鹉、白眼鹦哥和蓝黄金刚鹦鹉收集了47份粪便样本。所有样本均在鸟类排便时采集。使用ZR粪便DNA微量制备试剂盒（Zymo Research）进行DNA提取。通过靶向18S RNA基因的巢式PCR和扩增片段测序来筛查隐孢子虫。在蓝黄金刚鹦鹉（4份）和白眼鹦哥（1份）样本中发现隐孢子虫呈阳性（10.64%；5/47）。对扩增片段进行测序，结果显示与贝氏隐孢子虫的基因相似度为100%。
