丝氨醇核酸作为超灵敏分子信标的应用。
The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons.
作者信息
Murayama Keiji, Kashida Hiromu, Asanuma Hiroyuki
机构信息
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.
PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama, Japan.
出版信息
Methods Mol Biol. 2019;1973:261-279. doi: 10.1007/978-1-4939-9216-4_17.
Molecular beacons composed of the artificial serinol nucleic acid (SNA) have demonstrated utility as novel fluorescence probes for visualization of RNA in fixed cells using both conventional fluorescence in situ hybridization (FISH) and wash-free FISH protocols. The SNA molecular beacons have higher affinity for target RNA and greater sensitivity than molecular beacons composed of DNA. Here we describe facile synthesis of the SNA using a conventional DNA synthesizer and protocols for purification by PAGE and HPLC as well as methods for use of the SNA molecular beacon in FISH.
由人工丝氨酸核酸（SNA）组成的分子信标已被证明可作为新型荧光探针，用于在固定细胞中使用传统荧光原位杂交（FISH）和免洗FISH方案对RNA进行可视化。与由DNA组成的分子信标相比，SNA分子信标对靶RNA具有更高的亲和力和更高的灵敏度。在这里，我们描述了使用传统DNA合成仪简便合成SNA的方法，以及通过PAGE和HPLC进行纯化的方案，以及SNA分子信标在FISH中的使用方法。
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