Sezik Mekin, Köker Afşin, Özmen Özlem, Halıgür Mehmet, Kaşıkçı Duygu, Aydoğan Ahmet, Özatik Orhan
Süleyman Demirel University Faculty of Medicine, Department of Obstetrics and Gynecology, Isparta, Turkey.
Mehmet Akif Ersoy University Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, Burdur, Turkey.
Turk J Obstet Gynecol. 2019 Mar;16(1):41-49. doi: 10.4274/tjod.tjod.galenos.2019.92300. Epub 2019 Mar 27.
To define a novel experimental model with maternal intravenous (i.v.) granulocyte-colony stimulating factor (G-CSF) followed by a single- and high-dose of 20 mg intra-amniotic (IA) endotoxin to induce fetal brain injury in the preterm fetal goat.
Pregnant goats (n=4) were given 50 microg/day G-CSF into the maternal jugular vein through gestational days 110-115 (term, 150 days). At gestational day 115, 20 mg of IA endotoxin was administered. Following preterm delivery at day 120 by cesarean section umbilical cord, fetal lung and brain tissues were harvested for histopathology, immunohistochemistry, and electron microscopy. Inflammatory markers were evaluated in the amniotic fluid and fetal plasma.
Necrotizing funisitis with abundant leukocyte infiltration and fetal brain injury was induced in all the fetuses in the experimental group.
Maternal i.v. G-CSF for 5 days followed by 20 mg of IA endotoxin is a feasible caprine model to exacerbate intrauterine inflammation.
建立一种新的实验模型，即对孕羊静脉注射粒细胞集落刺激因子（G-CSF），随后单次高剂量羊膜腔内注射20 mg内毒素，以诱导早产山羊胎儿脑损伤。
4只孕羊在妊娠第110至115天（足月为150天）经母羊颈静脉给予50μg/天的G-CSF。在妊娠第115天，羊膜腔内注射20 mg内毒素。在第120天通过剖宫产取出脐带后早产，收集胎儿肺和脑组织进行组织病理学、免疫组织化学和电子显微镜检查。评估羊水和胎儿血浆中的炎症标志物。
实验组所有胎儿均出现伴有大量白细胞浸润的坏死性脐带炎和胎儿脑损伤。
孕羊静脉注射G-CSF 5天，随后羊膜腔内注射20 mg内毒素，是一种加剧宫内炎症的可行的山羊模型。
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