基于质谱的TRPP2磷酸化分析
Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation.
作者信息
Dumit Verónica I, Köttgen Michael, Hofherr Alexis
机构信息
Core Facility Proteomics, Center for Biological Systems Analysis (ZBSA), University of Freiburg, Freiburg, Germany.
Renal Division, Department of Medicine, Faculty of Medicine, Medical Center, University of Freiburg, Freiburg, Germany.
出版信息
Methods Mol Biol. 2019;1987:51-64. doi: 10.1007/978-1-4939-9446-5_4.
Differential phosphorylation of proteins is a key regulatory mechanism in biology. Immunoprecipitation-coupled mass spectrometry facilitates the targeted analysis of transient receptor ion potential channel polycystin-2 (TRPP2) phosphorylation. However, empirical testing is required to optimize experimental conditions for immunoprecipitation and mass spectrometry. Here, we present a detailed workflow for the reliable analysis of endogenous TRPP2 phosphorylation in differentiated renal epithelial cells.
蛋白质的差异磷酸化是生物学中的一种关键调节机制。免疫沉淀耦合质谱法有助于对瞬时受体离子电位通道多囊蛋白-2（TRPP2）的磷酸化进行靶向分析。然而，需要进行实证测试以优化免疫沉淀和质谱分析的实验条件。在此，我们展示了一种用于可靠分析分化肾上皮细胞中内源性TRPP2磷酸化的详细工作流程。
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