Tang Qiuxiang, He Guiqiang, Huang Jun, Wu Chongde, Jin Yao, Zhou Rongqing
College of Light Industry, Fermentation Engineering, Sichuan University, Chengdu, China.
Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu, China.
Front Microbiol. 2019 Apr 12;10:696. doi: 10.3389/fmicb.2019.00696. eCollection 2019.
is a fermentation starter used in the production of , which is also a traditional Chinese liquor. The quality and microbial community characteristics of is closely related with the yield and flavor feature of fresh . The present study aims to explore the mystery behind microbial diversity and volatiles of through polyphasic detection methods such as the Illumina MiSeq platform and the metabolite analyzing method. Results showed that differences in microbial community diversity among samples were significant. The hydrolytic ability was positively correlated with α- and β-diversity of bacteria, but negatively correlated with that of fungi. and were the dominant bacteria, while and were the dominant fungi. The abundance of bacteria in sample No3 ranged from 33.66 to 91.53%, while sample No4 the abundance of fungi ranged from 58.51 to 48.72%. The difference of microbial community diversity resulted in a discrepancy of volatile profiles and interaction relationship among the genus. Twenty-four dominant bacteria and seven dominant fungi were correlated with 20 different volatiles. This study provides a scientific perspective of the uniformity and stability of and might aid in controlling its manufacturing process.
是一种用于生产的发酵剂,也是一种中国传统白酒。的质量和微生物群落特征与新鲜的产量和风味特征密切相关。本研究旨在通过Illumina MiSeq平台和代谢物分析方法等多相检测方法探索背后的微生物多样性和挥发性物质的奥秘。结果表明,样品间微生物群落多样性差异显著。水解能力与细菌的α-多样性和β-多样性呈正相关,但与真菌的α-多样性和β-多样性呈负相关。和是优势细菌,而和是优势真菌。3号样品中细菌丰度范围为33.66%至91.53%,4号样品中真菌丰度范围为58.51%至48.72%。微生物群落多样性的差异导致了挥发性成分谱和属间相互作用关系的差异。24种优势细菌和7种优势真菌与20种不同的挥发性物质相关。本研究为的均匀性和稳定性提供了科学视角,并可能有助于控制其生产过程。