Derafshpour Leila, Saboory Ehsan, Vafaei Abbas Ali, Rashidy-Pour Ali, Roshan-Milani Shiva, Rasmi Yousef, Panahi Yousef, Sameni Hamidreza
Laboratory of Learning and Memory, Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran.
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Basic Clin Neurosci. 2019 Mar-Apr;10(2):119-135. doi: 10.32598/bcn.9.10.170. Epub 2019 Mar 1.
The long-term adverse effects of transient thyroid function abnormalities at birth on intellectual development are proven. The effect of exercise increases in the presence of sex hormones. The current study aimed at investigating the possibility that a combination of sex hormones and exercise has synergistic effects on neural plasticity in Transient Congenital Hypothyroidism (TCH) rats.
To induce hypothyroidism in the mothers, Propylthiouracil (PTU) was added to drinking water (100 mg/L) on the 6th day of gestation and continued until the 21 Postnatal Day. From Postnatal Day (PND) 28 to 47, the female and male pups received 17β-estradiol and testosterone, respectively. The mild treadmill exercise began 30 minutes after the sex hormones or vehicle administration. On PND 48, electrophysiological experiments were performed on brain slices.
Increase of Long-Term Potentiation (LTP) was observed in sedentary-non-hormone female rats of TCH group, compared with that of the control. The exercise enhanced LTP in control rats, but the hormones showed no significant effect. The effect of exercise and sex hormone was not significant in the TCH group. The combination of exercise and testosterone enhanced LTP in TCH male rats, while the combination of exercise and estradiol or each of them individually did not produce such an effect on LTP in TCH female rats.
The study findings showed an increase in excitatory transmission despite the returning of thyroid hormone levels to normal range in TCH female rats. Also a combination treatment including exercise and testosterone enhanced LTP in male rats of TCH group, which was a gender-specific event.
出生时短暂的甲状腺功能异常对智力发育的长期不良影响已得到证实。运动的效果在有性激素存在时会增强。本研究旨在探讨性激素与运动相结合对短暂先天性甲状腺功能减退症（TCH）大鼠神经可塑性产生协同作用的可能性。
为诱导母鼠甲状腺功能减退，在妊娠第6天向饮用水中添加丙硫氧嘧啶（PTU，100mg/L），并持续至出生后第21天。从出生后第28天至47天，雌性和雄性幼崽分别接受17β-雌二醇和睾酮。在给予性激素或赋形剂30分钟后开始轻度跑步机运动。在出生后第48天，对脑片进行电生理实验。
与对照组相比，TCH组久坐不动且未接受激素处理的雌性大鼠的长时程增强（LTP）增加。运动增强了对照组大鼠的LTP，但激素未显示出显著效果。在TCH组中，运动和性激素的作用不显著。运动与睾酮的组合增强了TCH雄性大鼠的LTP，而运动与雌二醇的组合或它们各自单独使用对TCH雌性大鼠的LTP均未产生这种效果。
研究结果表明，尽管TCH雌性大鼠的甲状腺激素水平已恢复至正常范围，但兴奋性传递仍有所增加。此外，包括运动和睾酮在内的联合治疗增强了TCH组雄性大鼠的LTP，这是一个性别特异性事件。
