College of Life and Health Science, Kaili University, Kaili City, Guizhou Province, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China.
PLoS One. 2019 Apr 29;14(4):e0216299. doi: 10.1371/journal.pone.0216299. eCollection 2019.
Blueberry is a kind of new rising popular perennial fruit with high healthful quality. It is of utmost importance to develop new blueberry varieties for different climatic zones to satisfy the demand of people in the world. Molecular marker assisted breeding is believed to be an ideal method for the development of new blueberry varieties for its shorter breeding cycle than the conventional breeding. Simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) markers are widely used molecular tools for marker assisted breeding, which could be detected at large scale by the transcriptome sequencing. Here, we sequenced the leaves transcriptome of 19 rabbiteye (Vaccinium ashei Reade), 13 southern highbush (Vaccinium. corymbosum L × native southern Vaccinium Spp) and 22 cultivars of northern highbush blueberry (Vaccinium corymbosum L) by using next generation sequencing technologies. A total of 80.825 Gb clean data with an average of about 12.525 million reads per cultivar were obtained. We assembled 58,968, 55,973 and 53,887 unigenes by using the clean data from rabbiteye, southern highbush and northern highbush blueberry cultivars, respectively. Among these unigenes, 3599, 3495 and 3513 unigenes were detected as candidate resistance genes in three blueberry crops. Moreover, we identified more than 8756, 9020, and 9198 SSR markers from these unigenes, and 7665, 4861, 13,063 SNPs from the annotated single copy unigenes, respectively. The results will be helpful for the molecular genetics and association analysis of blueberry and the basic molecular information of pest and disease resistance of blueberry, and would also offer huge number of molecular tools for the marker assisted breeding to produce blueberry cultivars with different adaptive characteristics.
蓝莓是一种新兴的流行的多年生水果，具有很高的健康品质。开发适合不同气候带的蓝莓新品种对于满足世界人民的需求至关重要。与传统育种相比，分子标记辅助育种被认为是开发蓝莓新品种的理想方法，因为它的育种周期更短。简单重复序列（SSR）和单核苷酸多态性（SNP）标记是广泛用于标记辅助育种的分子工具，可以通过转录组测序进行大规模检测。在这里，我们通过下一代测序技术对 19 个兔眼（Vaccinium ashei Reade）、13 个南方高丛（Vaccinium. corymbosum L × 本地南方 Vaccinium Spp）和 22 个北方高丛蓝莓（Vaccinium corymbosum L）的叶片转录组进行了测序。总共获得了 80.825 Gb 清洁数据，每个品种的平均读取量约为 1252.5 万条。我们分别使用来自兔眼、南方高丛和北方高丛蓝莓品种的清洁数据组装了 58968、55973 和 53887 个 unigenes。在这些 unigenes中，3599、3495 和 3513 个 unigenes被检测为三种蓝莓作物的候选抗性基因。此外，我们从这些 unigenes中鉴定出了 8756 个以上、9020 个和 9198 个 SSR 标记，从注释的单拷贝 unigenes中鉴定出了 7665 个、4861 个和 13063 个 SNP。这些结果将有助于蓝莓的分子遗传学和关联分析，以及蓝莓病虫害抗性的基础分子信息，也将为标记辅助育种提供大量的分子工具，以培育具有不同适应性特征的蓝莓品种。
