Nistal M, Santamaria L, Paniagua R, Regadera J
Acta Morphol Hung. 1986;34(1-2):107-15.
The testicular connective tissue of patients with alcoholic and non-alcoholic cirrhosis was studied by Picrosirius staining for collagen fibres and electron microscopy, and then compared with the connective tissue of normal testes. Under polarized light, the interstitial connective tissue in the testes of patients with alcoholic and non-alcoholic cirrhosis exhibited a green birefringence which did not appear in normal testes, and the orange birefringence characteristic of the tunica propria in the testes of both cirrhotic and normal males was less developed in cirrhosis. This suggests that a connective tissue, consisting of smaller and less ordered collagen fibre bundles than those in the tunica propria is formed in the testicular interstitium of cirrhotic males, and the collagen fibre bundles of the tunica propria are less developed than in normal testes. The appearance of connective tissue was associated with a decrease in the number of mast cells in the testes of cirrhotic males suggesting the involvement of mast cells in the synthesis, packing, and organization of collagen fibres. The cause of the decrease in mast cell numbers may be related to hormone alterations, in particular testosterone deficiency.
通过对胶原纤维进行天狼星红染色和电子显微镜观察,研究了酒精性和非酒精性肝硬化患者的睾丸结缔组织,然后将其与正常睾丸的结缔组织进行比较。在偏振光下,酒精性和非酒精性肝硬化患者睾丸中的间质结缔组织呈现出绿色双折射,而正常睾丸中未出现这种情况,并且在肝硬化患者中,无论是肝硬化男性还是正常男性睾丸中固有膜特有的橙色双折射都不明显。这表明,在肝硬化男性的睾丸间质中形成了一种结缔组织,其胶原纤维束比固有膜中的更小且排列更无序,并且固有膜的胶原纤维束比正常睾丸中的发育更差。结缔组织的出现与肝硬化男性睾丸中肥大细胞数量的减少有关,这表明肥大细胞参与了胶原纤维的合成、堆积和组织。肥大细胞数量减少的原因可能与激素变化有关,尤其是睾酮缺乏。