National R＆D Center For Citrus Preservation, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
Food Chem. 2019 Sep 15;292:314-324. doi: 10.1016/j.foodchem.2019.04.009. Epub 2019 Apr 2.
Little is known about the variations of fresh fruit biomembrane and its physiological and biochemical characteristics during storage. A navel orange mutant 'Gannan No.1' (Citrus sinensis Osbeck) showed higher membrane stability and titratable acid while lower calyx senescence compared with wild-type 'Newhall'. The membrane damage was significantly reduced in 'Gannan No.1' under 10% polyethylene-glycol (41.16% vs. 8.77%) and 30% polyethylene-glycol (52.59% vs.16.11%) treatments on day 45 after harvest. Consistently, membrane electrolyte leakage and malondialdehyde were significantly decreased in 'Gannan No.1', and superoxide dismutase and glutathione reductase were activated. A metabolic analysis was performed to evaluate membrane fatty acid unsaturation and peroxidation. Linolenic acid and hexadecylenic acid contributed to the higher degree of unsaturated fatty acids in 'Gannan No.1'. Furthermore, 'Gannan No.1' accumulated stress-resistant metabolites such as proline, α-tocopherol and glutathione. Correlation analysis of membrane homeostasis indexes with quality parameters showed the importance of biomembrane stability in maintaining citrus fruit quality.
关于鲜果皮生物膜的变化及其在贮藏过程中的生理生化特性，目前知之甚少。与野生型“纽荷尔”（Newhall）相比，脐橙突变体“赣南 1 号”（Citrus sinensis Osbeck）表现出更高的膜稳定性和可滴定酸，以及更低的花萼衰老。在收获后第 45 天，10%聚乙二醇（41.16%对 8.77%）和 30%聚乙二醇（52.59%对 16.11%）处理下，“赣南 1 号”的膜损伤显著减少。同样，膜电解质渗漏和丙二醛在“赣南 1 号”中显著降低，超氧化物歧化酶和谷胱甘肽还原酶被激活。进行了代谢分析以评估膜脂肪酸的不饱和度和过氧化作用。亚麻酸和十六烯酸有助于“赣南 1 号”中不饱和脂肪酸的含量更高。此外，“赣南 1 号”积累了具有抗应激作用的代谢物，如脯氨酸、α-生育酚和谷胱甘肽。膜内稳性指标与品质参数的相关分析表明，生物膜稳定性在保持柑橘果实品质方面的重要性。
