醋酸甲羟孕酮会影响斑马鱼的性别分化和精子发生。
Medroxyprogesterone acetate affects sex differentiation and spermatogenesis in zebrafish.
机构信息
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou, 510006, China; State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.
SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou, 510006, China.
出版信息
Aquat Toxicol. 2019 Jul;212:70-76. doi: 10.1016/j.aquatox.2019.04.014. Epub 2019 Apr 25.
Medroxyprogesterone acetate (MPA) is a widely used synthetic progestin and it has been frequently detected in aquatic environments. However, its effects on aquatic organisms remain largely unknown. Here we investigated the chronic effects of MPA on sex differentiation and gonad development in zebrafish. Zebrafish larvae at 20 days post fertilization (dpf) were exposed to 4.32, 42.0, and 424 ng L of MPA until they reached 140 dpf. The results showed that chronic exposure to 42.0 ng L of MPA caused 60% proportion of males as well as significant up-regulation of dmrt1 (˜1.79 fold) and hsd17b3 (˜1.92 fold). Histological analysis showed MPA significantly increased the frequency of immature spermatocytes accompanied with the increased transcription of dmrt1 (˜2.06 fold) and ar (˜1.73 fold) in the testes. Meanwhile, MPA exposure significantly increased the transcription of lhb at all exposure concentrations in the males. In contrast, it significantly suppressed the transcription of lhb (˜-8.06-fold) and fshb (˜-6.35-fold) at 42.0 ng L in the females. Collectively our results demonstrated that MPA had androgenic activity, and could affect sex differentiation and spermatogenesis in zebrafish at environmentally relevant concentrations. The findings from this study suggest that MPA in the aquatic environment may pose potential androgenic risks to fish populations.
醋酸甲羟孕酮（MPA）是一种广泛使用的合成孕激素，它经常在水生环境中被检测到。然而，它对水生生物的影响在很大程度上仍然未知。在这里，我们研究了 MPA 对斑马鱼性别分化和性腺发育的慢性影响。斑马鱼幼虫在受精后 20 天（dpf）暴露于 4.32、42.0 和 424ng/L 的 MPA 中，直到它们达到 140 dpf。结果表明，慢性暴露于 42.0ng/L 的 MPA 导致 60%的雄性比例以及 dmrt1（约 1.79 倍）和 hsd17b3（约 1.92 倍）的显著上调。组织学分析表明，MPA 显著增加了不成熟精母细胞的频率，同时睾丸中 dmrt1（约 2.06 倍）和 ar（约 1.73 倍）的转录也增加了。同时，在所有暴露浓度下，MPA 暴露显著增加了雄性中 lhb 的转录。相比之下，它在雌性中 42.0ng/L 时显著抑制了 lhb（约-8.06 倍）和 fshb（约-6.35 倍）的转录。总之，我们的研究结果表明，MPA 具有雄激素活性，并能以环境相关浓度影响斑马鱼的性别分化和精子发生。本研究结果表明，水生环境中的 MPA 可能对鱼类种群构成潜在的雄激素风险。
Zotero 插件