用人蛋黄改良的冷冻保护剂对人类配子进行冷冻保存。
Human Gametes Cryopreservation with Cryoprotectant Modified by Egg Yolk.
作者信息
Grigorieva A, Simonenko E, Garmaeva S, Mironova A, Yakovenko S
机构信息
Lomonosov Moscow State University, Physical Department, GSP-1, Leninskie gory, Moscow, Russia.
Semenov Institute of Chemical Physics, 4 Kosygina street, building 1, Moscow, Russia.
出版信息
Cryo Letters. 2019 May/Jun;40(3):187-192.
BACKGROUND
The modification of cryoprotectants with an egg yolk is an effective method to improve cell survival.
OBJECTIVE
This study is dedicated to the comparison of four variants of cryoprotectants modified with egg yolk (commercially available and custom developed with yolk emulsion at 5.6 mg ml) and with lecithin.
METHODS
Cryoprotectant effectiveness was evaluated by cytotoxicity and vital screen tests, and by the determination of sperm motility index.
RESULTS
A simple analytical model has been created to determine an approximate concentration of cholesterol in the solution sufficient to stabilize cellular membranes. It was shown that the average fractions of living cells, motility and membrane integrity were higher for our modification.
CONCLUSION
We conclude that phosphatidylcholine (1.5 mg ml) contributes to the cryoprotective action of egg yolk, but doesnt define it entirely. The cryoprotective effect of egg yolk on plasma membranes is complex, and isnt caused by the action of one of its components.
背景
用蛋黄对冷冻保护剂进行改性是提高细胞存活率的有效方法。
目的
本研究致力于比较四种用蛋黄改性的冷冻保护剂变体（市售的以及用5.6毫克/毫升蛋黄乳液定制开发的）和用卵磷脂改性的冷冻保护剂。
方法
通过细胞毒性和活力筛选试验以及精子活力指数的测定来评估冷冻保护剂的有效性。
结果
建立了一个简单的分析模型来确定溶液中足以稳定细胞膜的胆固醇的近似浓度。结果表明，我们改性后的活细胞、活力和膜完整性的平均比例更高。
结论
我们得出结论，磷脂酰胆碱（1.5毫克/毫升）有助于蛋黄的冷冻保护作用，但不能完全决定它。蛋黄对质膜的冷冻保护作用是复杂的，不是由其一种成分的作用引起的。
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