Detection of cell-free circulating BRAF by droplet digital polymerase chain reaction in patients with and without melanoma under dermatological surveillance.

BACKGROUND

The p.V600E mutation in the BRAF protein is the most frequent mutation in cutaneous melanoma and is a recurrent alteration found in common benign naevi. Analysis of the cell-free BRAF c.1799T>A, p.V600E mutation (cfBRAF ) in plasma has emerged as a biomarker for monitoring prognosis and treatment response in patients with melanoma.

OBJECTIVES

To quantify cfBRAF levels in plasma from patients with melanoma and from patients without melanoma undergoing regular follow-up of their melanocytic lesions, in order to assess the clinical significance of the test.

METHODS

We quantified cfBRAF by droplet digital polymerase chain reaction in plasma from 146 patients without melanoma undergoing continuous dermatological screening, from 26 stage III and seven stage IV patients with BRAF-mutant melanoma, and from 32 patients with melanoma who were free of disease for 3 or more years.

RESULTS

Among disease-free patients and individuals without melanoma, 52% presented a high naevus count (> 50) and 49% had clinically atypical naevi. cfBRAF was detected in 71% of patients with stage IV melanoma and 15% with stage III, and in 1·4% of individuals without melanoma. No cfBRAF mutation was detected in disease-free patients with melanoma. Individuals without melanoma had lower cfBRAF levels than patients with melanoma. We established a variant allelic frequency of 0·26% or 5 copies mL of cfBRAF as the optimal cutoff value for identifying patients with melanoma with > 99% specificity.

CONCLUSIONS

This study suggests that naevus-related factors do not influence the detection of cfBRAF in individuals without melanoma, and supports the clinical diagnostic value of plasma cfBRAF quantification in patients with melanoma. What's already known about this topic? The analysis of the BRAF c.1799T>A (p.V600E) mutation in cell-free (cf)DNA has emerged as a potential biomarker for monitoring prognosis and treatment response in patients with metastatic BRAF melanoma. The BRAF alteration is a common genetic alteration found in benign proliferations such as melanocytic naevi. No information exists about the impact of the number of common acquired naevi or the presence of clinically atypical naevi in cfBRAF detection in an individual. What does this study add? The cfBRAF mutation is detected in plasma from a reduced number of individuals without melanoma undergoing continuous dermatological follow-up. A high number of naevi or the presence of clinically atypical naevi are factors that do not influence cfBRAF detection in an individual. Both total cfBRAF concentration and cfBRAF frequency are effective biomarkers in patients with advanced melanoma but not in patients at early stages or with micrometastases. What is the translational message? Detection of cfBRAF in an individual is not influenced by naevus-related factors. cfBRAF is a robust and reliable biomarker that can be used in dermatological surveillance programmes.