Cdc42 调控扁形动物表皮再生和稳态所必需的凋亡细胞死亡。

Cdc42 regulate the apoptotic cell death required for planarian epidermal regeneration and homeostasis.

机构信息

School of LifeSciences, Zhengzhou University, Zhengzhou, Henan, China.

Department of Biochemistry and Molecular Pharmacology, New York University, School of Medicine, NY, USA.

出版信息

Int J Biochem Cell Biol. 2019 Jul;112:107-113. doi: 10.1016/j.biocel.2019.05.008. Epub 2019 May 15.

DOI:10.1016/j.biocel.2019.05.008

PMID:31102665

Abstract

Rho GTPases have been shown previously to play important roles in several cellular processes by regulating the organization of the actin and microtubule cytoskeletons. However, the mechanisms of Rho GTPases that integrate the cellular responses during regeneration have not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage development in vivo. Here, we focus on cdc42, which is an extensively characterized member among Rho GTPases. We found that cdc42 is required for the maintenance of epidermal lineage. Cdc42 RNAi induced a sustained increased of cell death and led to a loss of the mature epidermal cells but without affected cell division. Our results indicate that cdc42 function as an inhibitor to block the excessive apoptotic cell death in planarian epidermal regeneration and homeostasis.

摘要

先前的研究表明，Rho GTPases 通过调节肌动蛋白和微管细胞骨架的组织，在几个细胞过程中发挥重要作用。然而，整合再生过程中细胞反应的 Rho GTPases 机制尚未得到彻底阐明。扁形动物（Planarian），含有大量成年体干细胞（成体神经干细胞，neoblasts），是研究体内干细胞谱系发育的独特模型。在这里，我们重点关注 Rho GTPases 中广泛研究的 cdc42。我们发现 cdc42 对于表皮谱系的维持是必需的。cdc42 RNAi 诱导持续增加的细胞死亡，并导致成熟的表皮细胞丢失，但不影响细胞分裂。我们的结果表明，cdc42 作为一种抑制剂发挥作用，阻止扁形动物表皮再生和动态平衡中过度的凋亡细胞死亡。