Gi信号转导蛋白α亚基的人源cDNA克隆。
Human cDNA clones for an alpha subunit of Gi signal-transduction protein.
作者信息
Bray P, Carter A, Guo V, Puckett C, Kamholz J, Spiegel A, Nirenberg M
出版信息
Proc Natl Acad Sci U S A. 1987 Aug;84(15):5115-9. doi: 10.1073/pnas.84.15.5115.
Two cDNA clones were obtained from a lambda gt11 cDNA human brain library that correspond to alpha i subunits of G signal-transduction proteins (where alpha i subunits refer to the alpha subunits of G proteins that inhibit adenylate cyclase). The nucleotide sequence of human brain alpha i is highly homologous to that of bovine brain alpha i [Nukada, T., Tanabe, T., Takahashi, H., Noda, M., Haga, K., Haga, T., Ichiyama, A., Kangawa, K., Hiranaga, M., Matsuo, H. & Numa, S. (1986) FEBS Lett. 197, 305-310] and the predicted amino acid sequences are identical. However, human and bovine brain alpha i cDNAs differ significantly from alpha i cDNAs from human monocytes, rat glioma, and mouse macrophages in amino acid (88% homology) and nucleotide (71-75% homology) sequences. In addition, the nucleotide sequences of the 3' untranslated regions of human and bovine brain alpha i cDNAs differ markedly from the sequences of human monocyte, rat glioma, and mouse macrophage alpha i cDNAs. These results suggest there are at least two classes of alpha i mRNA.
从一个λgt11 cDNA人脑文库中获得了两个cDNA克隆，它们对应于G信号转导蛋白的αi亚基（其中αi亚基指的是抑制腺苷酸环化酶的G蛋白的α亚基）。人脑αi的核苷酸序列与牛脑αi的核苷酸序列高度同源[Nukada, T., Tanabe, T., Takahashi, H., Noda, M., Haga, K., Haga, T., Ichiyama, A., Kangawa, K., Hiranaga, M., Matsuo, H. & Numa, S. (1986) FEBS Lett. 197, 305 - 310]，并且预测的氨基酸序列是相同的。然而，人脑和牛脑αi cDNA在氨基酸序列（88%同源性）和核苷酸序列（71 - 75%同源性）上与人单核细胞、大鼠胶质瘤和小鼠巨噬细胞的αi cDNA有显著差异。此外，人脑和牛脑αi cDNA的3'非翻译区的核苷酸序列与人类单核细胞、大鼠胶质瘤和小鼠巨噬细胞αi cDNA的序列明显不同。这些结果表明至少存在两类αi mRNA。
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