人诱导多能干细胞来源的定形内胚层批量培养及肝脏分化

Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation.

作者信息

Palakkan Anwar A, Nanda Jyoti, Ross James A

机构信息

Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK.

出版信息

Methods Mol Biol. 2019;1994:41-53. doi: 10.1007/978-1-4939-9477-9_4.

DOI:10.1007/978-1-4939-9477-9_4

PMID:31124103

Abstract

We have developed a method to bulk culture definitive endoderm cells generated from human iPSCs which can be stored and differentiated to hepatocytes. Human iPSC-derived definitive endoderm cells were sorted based on the expression of CXCR4. The sorted cells were able to proliferate for extended periods and can be cryopreserved. The definitive endoderm cells were subsequently utilized to generate functional hepatocytes expressing albumin and α-fetoprotein in different multiwell formats. This provides a method to reliably produce more consistent hepatocytes in greater quantities and has enabled the development of high-throughput screening strategies.

摘要

我们已经开发出一种方法，用于批量培养源自人类诱导多能干细胞（iPSC）的定形内胚层细胞，这些细胞可以储存并分化为肝细胞。基于CXCR4的表达对人iPSC衍生的定形内胚层细胞进行分选。分选后的细胞能够长时间增殖并且可以冷冻保存。随后，利用定形内胚层细胞以不同的多孔板形式生成表达白蛋白和甲胎蛋白的功能性肝细胞。这提供了一种可靠地大量生产更一致肝细胞的方法，并推动了高通量筛选策略的发展。