Palakkan Anwar A, Nanda Jyoti, Ross James A
Tissue Injury and Repair Group, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2019;1994:41-53. doi: 10.1007/978-1-4939-9477-9_4.
We have developed a method to bulk culture definitive endoderm cells generated from human iPSCs which can be stored and differentiated to hepatocytes. Human iPSC-derived definitive endoderm cells were sorted based on the expression of CXCR4. The sorted cells were able to proliferate for extended periods and can be cryopreserved. The definitive endoderm cells were subsequently utilized to generate functional hepatocytes expressing albumin and α-fetoprotein in different multiwell formats. This provides a method to reliably produce more consistent hepatocytes in greater quantities and has enabled the development of high-throughput screening strategies.
我们已经开发出一种方法，用于批量培养源自人类诱导多能干细胞（iPSC）的定形内胚层细胞，这些细胞可以储存并分化为肝细胞。基于CXCR4的表达对人iPSC衍生的定形内胚层细胞进行分选。分选后的细胞能够长时间增殖并且可以冷冻保存。随后，利用定形内胚层细胞以不同的多孔板形式生成表达白蛋白和甲胎蛋白的功能性肝细胞。这提供了一种可靠地大量生产更一致肝细胞的方法，并推动了高通量筛选策略的发展。
