Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci. 2019 May 23;20(10):2538. doi: 10.3390/ijms20102538.
The RNA silencing pathways modulate responses to certain stresses, and can be partially tuned by several hormones such as salicylic acid (SA) and abscisic acid (ABA). Although SA and ABA are often antagonistic and often modulate different stress responses, they have similar effects on virus resistance, which are partially achieved through the antiviral RNA silencing pathway. Whether they play similar roles in regulating the RNA silencing pathway is unclear. By employing coexpression and promoter analyses, we found that some ABA- and SA-related transcription factors (TFs) are coexpressed with several , , and genes, and have multiple binding sites for the identified TFs in the queried promoters. ABA and SA are antagonistic with respect to the expression of and because ABA was able to induce these genes only in the SA mutant. Nevertheless, both hormones showed similarities in the regulation of other genes, for example, the induction of by ABA was SA-dependent, indicating that ABA acts upstream of SA in this regulation. We inferred that the similar effects of ABA and SA on some genes resulted in the redundancy of their roles in resistance to bamboo mosaic virus, but that the two hormones are antagonistic with respect to other genes unrelated to their biosynthesis pathways.
RNA 沉默途径调节对某些应激的反应，并且可以被几种激素如水杨酸（SA）和脱落酸（ABA）部分调节。尽管 SA 和 ABA 通常是拮抗的，并且通常调节不同的应激反应，但它们对病毒抗性具有相似的影响，这部分是通过抗病毒 RNA 沉默途径实现的。它们在调节 RNA 沉默途径中是否发挥相似的作用尚不清楚。通过共表达和启动子分析，我们发现一些 ABA 和 SA 相关的转录因子（TFs）与几个 、 和 基因共表达，并且在所查询的启动子中具有多个鉴定的 TF 的结合位点。ABA 和 SA 在 和 的表达上是拮抗的，因为 ABA 只能在 SA 突变体中诱导这些基因。然而，两种激素在其他基因的调控上表现出相似性，例如，ABA 诱导 的表达依赖于 SA，表明 ABA 在这种调控中位于 SA 的上游。我们推断，ABA 和 SA 对一些基因的相似影响导致了它们在抵抗竹嵌纹病毒中的作用冗余，但这两种激素在与它们的生物合成途径无关的其他基因上是拮抗的。
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