小梁网细胞内脂质代谢物转运的评估。

Assessment of Transport of Lipid Metabolites Within Trabecular Meshwork Cells.

作者信息

Toglia Daphne, Dancausse Stephanie, Kalahasty Karthik, Alfonso Bryan, Bhattacharya Sanjoy K

机构信息

Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

出版信息

Methods Mol Biol. 2019;1996:187-197. doi: 10.1007/978-1-4939-9488-5_16.

DOI:10.1007/978-1-4939-9488-5_16

PMID:31127557

Abstract

Lipids from trabecular meshwork (TM) cells are of particular interest to ophthalmological researchers as a therapeutic target for lowering intraocular pressure (IOP) in glaucomatous eyes. Fluorescence-based lipid transport assays (FBLTA) and immunocytochemistry (ICC) are dynamic fluorescence analysis techniques that allow for quantitative and qualitative comparisons, respectively, between multiple samples. Here we describe methods for FBLTA, ICC, and mass spectroscopy designed to measure the kinetics and localization of lipid metabolites within the trabecular meshwork.

摘要

小梁网（TM）细胞中的脂质作为降低青光眼患者眼压的治疗靶点，受到眼科研究人员的特别关注。基于荧光的脂质转运分析（FBLTA）和免疫细胞化学（ICC）是动态荧光分析技术，分别允许对多个样品进行定量和定性比较。在这里，我们描述了用于FBLTA、ICC和质谱分析的方法，这些方法旨在测量小梁网内脂质代谢物的动力学和定位。