非洲爪蟾卵提取物中质粒结合蛋白的染色质免疫沉淀（ChIP）
Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.
作者信息
Wolfe Kelly B, Long David T
机构信息
Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA.
出版信息
Methods Mol Biol. 2019;1999:173-184. doi: 10.1007/978-1-4939-9500-4_10.
Abstract
Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.
摘要
非洲爪蟾卵提取物提供了一个无细胞系统来分析染色质生物学的各个方面。在这里，我们描述了一种改良的染色质免疫沉淀（ChIP）方法，用于检测蛋白质与提取物中孵育的质粒DNA的相互作用。ChIP与非洲爪蟾卵提取物的结合提供了一种高度通用且易于处理的方法，能够在很大的空间和时间细节上分析动态的蛋白质-DNA相互作用。
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