Gali Himabindu, Mason-Osann Emily, Flynn Rachel Litman
Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, Boston, MA, USA.
Department of Medicine, Cancer Center, Boston University School of Medicine, Boston, MA, USA.
Methods Mol Biol. 2019;1999:319-325. doi: 10.1007/978-1-4939-9500-4_22.
The ability to analyze individual DNA fibers undergoing active DNA synthesis has emerged as a powerful technique in the field of DNA replication. Much of the initial analysis has focused on replication throughout the genome. However, more recent advancements in this technique have allowed for the visualization of replication patterns at distinct loci or regions within the genome. This type of locus-specific resolution will greatly enhance our understanding of the dynamics of DNA replication in regions that provide a challenge to the replication machinery. Here, we describe a protocol that will allow for the visualization of DNA replication through one of the most structurally complex regions in the human genome, the telomeric DNA.
分析正在进行活跃DNA合成的单个DNA纤维的能力，已成为DNA复制领域一项强大的技术。最初的分析大多集中在全基因组的复制。然而，这项技术最近的进展使得人们能够可视化基因组内不同位点或区域的复制模式。这种位点特异性分辨率将极大地增进我们对那些给复制机制带来挑战的区域中DNA复制动态的理解。在此，我们描述一种方案，该方案将通过人类基因组中结构最复杂的区域之一——端粒DNA，实现对DNA复制的可视化。
Methods Mol Biol. 2019
Methods Mol Biol. 2017
Nucleic Acids Res. 1998-8-15
Methods Mol Biol. 2011
Biosci Biotechnol Biochem. 2007-2
Methods Mol Biol. 2017
Zotero 插件