高特异性和敏感性的抗委内瑞拉类圆线虫IgY抗体应用于人类类圆线虫病免疫诊断。
Highly specific and sensitive anti-Strongyloides venezuelensis IgY antibodies applied to the human strongyloidiasis immunodiagnosis.
作者信息
de Faria Lucas S, de Souza Dayane L N, Ribeiro Raphaella P, de Sousa José Eduardo N, Borges Isabela P, Ávila Veridiana M R, Ferreira-Júnior Álvaro, Goulart Luiz Ricardo, Costa-Cruz Julia M
机构信息
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais 38400-902, Brazil.
Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais 38400-902, Brazil.
出版信息
Parasitol Int. 2019 Oct;72:101933. doi: 10.1016/j.parint.2019.101933. Epub 2019 May 23.
Due to the epidemiological problem of the neglected condition of human strongyloidiasis, rapid and effective diagnosis is extremely important, with the development of new diagnostic tools being essential to reduce infections and chronic cases. Avian immunoglobulin Y (IgY) technology is an alternative for antibody production that has high specificity and profitability. This study aimed to produce and fractionate IgY antibodies from the egg yolks of hens that were immunized with the total antigenic extracts of Strongyloides venezuelensis infectious filariform larvae (iL3) and parthenogenetic females (pF). IgY antibodies were then evaluated by their recognition of antigenic proteins, evolutive helminth forms, and serological diagnosis of human strongyloidiasis by the detection of immune complexes in serum samples. Egg yolks were fractionated to obtain IgY antibodies by thiophilic interaction chromatography. Immune complex detection in serum samples showed diagnostic values for anti-iL3 IgY and anti-pF IgY antibodies at 95.56% and 88.89% sensitivity and 95.56% and 91.11% specificity, respectively. Therefore, IgY technology is a promising tool for the detection of blood circulating Strongyloides antigens, with possible application as a serological diagnostic method.
由于人类类圆线虫病被忽视状况所带来的流行病学问题，快速有效的诊断极为重要，开发新的诊断工具对于减少感染和慢性病例至关重要。禽免疫球蛋白Y（IgY）技术是一种生产抗体的替代方法，具有高特异性和高效益。本研究旨在从用委内瑞拉类圆线虫感染性丝状幼虫（iL3）和孤雌生殖雌虫（pF）的总抗原提取物免疫的母鸡蛋黄中生产和分离IgY抗体。然后通过IgY抗体对抗原蛋白的识别、蠕虫发育形式以及通过检测血清样本中的免疫复合物对人类类圆线虫病进行血清学诊断来评估IgY抗体。通过嗜硫亲和色谱法分离蛋黄以获得IgY抗体。血清样本中的免疫复合物检测显示，抗iL3 IgY和抗pF IgY抗体的诊断价值分别为敏感性95.56%和88.89%，特异性95.56%和91.11%。因此，IgY技术是检测血液循环中类圆线虫抗原的一种有前景的工具，有可能作为一种血清学诊断方法应用。
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