桃乙烯响应因子 PpeERF2 在果实成熟过程中抑制 ABA 生物合成和细胞壁降解基因的表达。
Peach ethylene response factor PpeERF2 represses the expression of ABA biosynthesis and cell wall degradation genes during fruit ripening.
机构信息
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China; Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China.
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China.
出版信息
Plant Sci. 2019 Jun;283:116-126. doi: 10.1016/j.plantsci.2019.02.009. Epub 2019 Mar 12.
Ethylene response factors (ERFs) are known to regulate fruit ripening. However, the ERF regulatory networks are not clear. In this study, we have shown that peach (Prunus persica) PpeERF2 regulates fruit ripening through suppressing the expression of two ABA biosynthesis genes (PpeNCED2, PpeNCED3) and a cell wall degradation gene (PpePG1). The transcript levels of PpeERF2 in fruit were opposite to that of PpeNCED2, PpeNCED3 and PpePG1 during ripening and in response to various ripening treatments. PpeERF2 was found to bind to the PpeNCED2, PpeNCED3 and PpePG1 promotors as demonstrated by yeast one-hybrid (Y1H) and EMSA assays; and further found to repress the promoter activities of the three genes in tobacco leaf tissues after Agrobacterium infiltration. Taken together, these results provide new information for a better understanding of the crosstalk network between ethylene signaling, cell wall degradation and ABA biosynthesis during fruit ripening.
乙烯响应因子（ERFs）被认为可以调节果实成熟。然而，ERF 的调控网络尚不清楚。在这项研究中，我们表明，桃（Prunus persica）PpeERF2 通过抑制两个 ABA 生物合成基因（PpeNCED2、PpeNCED3）和一个细胞壁降解基因（PpePG1）的表达来调节果实成熟。在成熟过程中和对各种成熟处理的响应中，PpeERF2 在果实中的转录水平与 PpeNCED2、PpeNCED3 和 PpePG1 的转录水平相反。酵母单杂交（Y1H）和 EMSA 实验表明，PpeERF2 被发现与 PpeNCED2、PpeNCED3 和 PpePG1 的启动子结合；并且在农杆菌浸润烟草叶片组织后，进一步发现其可以抑制这三个基因的启动子活性。综上所述，这些结果为更好地理解乙烯信号、细胞壁降解和 ABA 生物合成之间的互作网络提供了新的信息。
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