从鼻旁粘膜中纯化的组织型纤溶酶原激活剂的一些化学性质。

Some chemical properties of tissue plasminogen activator purified from paranasal mucous membrane.

作者信息

Kosugi T, Nakamura M, Tsukayama T, Haraguchi S, Noda Y

出版信息

Rhinology. 1987 Jun;25(2):129-32.

Abstract

Plasminogen activator (PA) was purified from an acetone powder preparation of paranasal mucous membrane with chronic sinusitis, and some chemical properties of the purified PA were investigated in this paper. Zn-imminodiacetate affinity chromatography, lysine sepharose affinity chromatography and ultrafiltration for concentrating a PA fraction were consecutively performed to purify the PA from the acetone powder preparation. Finally, gel filtration was performed using Sephacryl S-200 in order to estimate the molecular weight of the purified PA. The purified PA in this experiment showed a stronger affinity to fibrin than urokinase did. The molecular weight of the purified PA was estimated to be 65,000 to 70,000 daltons as determined by Sephacryl S-200 gel filtration. The Km of the purified PA was 0.11 mM. From these results, it is apparent that the PA purified from an acetone powder preparation of paranasal mucous membrane belongs to the class of tissue type plasminogen activators (t-PA).

摘要

从患有慢性鼻窦炎的鼻旁黏膜丙酮粉制剂中纯化出纤溶酶原激活剂（PA），并对纯化后的PA的一些化学性质进行了研究。依次进行锌-亚氨基二乙酸亲和层析、赖氨酸琼脂糖亲和层析和超滤浓缩PA组分，以从丙酮粉制剂中纯化PA。最后，使用Sephacryl S-200进行凝胶过滤，以估计纯化后PA的分子量。本实验中纯化的PA对纤维蛋白的亲和力比尿激酶更强。通过Sephacryl S-200凝胶过滤测定，纯化后PA的分子量估计为65,000至70,000道尔顿。纯化后PA的Km为0.11 mM。从这些结果来看，显然从鼻旁黏膜丙酮粉制剂中纯化出的PA属于组织型纤溶酶原激活剂（t-PA）类别。