van de Voorde H, van Dijck P, Reybrouck G
Zentralbl Bakteriol Mikrobiol Hyg B Umwelthyg Krankenhaushyg Arbeitshyg Prav Med. 1987 May;184(2):160-6.
In order to standardize antifungal disinfectant testing using Candida albicans as a test organism, the morphology of four type strains of C. albicans DSM 1836, ATCC 10231, CNCM 1180-79 and CBS 562, grown on sixteen different media was determined. The incubation was carried out at 28 degrees C and 37 degrees C. The yeast phase was obtained predominantly on TSA medium with all strains. Mixed phases, i. e. true mycelium and yeast cells, were easily obtained in TSB in shaking flasks for three type strains but not for CBS 562. True mycelium was formed on corn meal media (CMA) for all strains, but the growth was very poor. The morphology of the growth was determined more by the type of strain than by the nutritive medium. Therefore C. albicans ATCC 10231 grown on either TSA or CMA was used as a test organism. Its resistance was determined towards four disinfectant standards: benzalkonium chloride, mercuric chloride, phenol and o-phenylphenol. No difference in sensitivity was found for the former disinfectant, but yeast cells grown on TSA were more resistant than C. albicans cultures on CMA in case of mercuric chloride, phenol and o-phenylphenol.
为了规范以白色念珠菌作为测试微生物的抗真菌消毒剂测试，测定了白色念珠菌DSM 1836、ATCC 10231、CNCM 1180 - 79和CBS 562这四株标准菌株在16种不同培养基上生长的形态。培养在28摄氏度和37摄氏度下进行。所有菌株在TSA培养基上主要获得酵母相。对于三株标准菌株，在摇瓶中的TSB培养基中很容易获得混合相，即真正的菌丝体和酵母细胞，但CBS 562菌株除外。所有菌株在玉米粉培养基（CMA）上都形成了真正的菌丝体，但生长非常差。生长形态更多地由菌株类型而非营养培养基决定。因此，以在TSA或CMA上生长的白色念珠菌ATCC 10231作为测试微生物。测定了其对四种消毒剂标准品的抗性：苯扎氯铵、氯化汞、苯酚和邻苯基苯酚。对于前一种消毒剂，未发现敏感性差异，但在氯化汞、苯酚和邻苯基苯酚的情况下，在TSA上生长的酵母细胞比在CMA上的白色念珠菌培养物更具抗性。
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