School of Public Health and Zoonoses, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India 141004.
FEMS Microbiol Lett. 2019 May 1;366(9). doi: 10.1093/femsle/fnz111.
Listeria monocytogenes is recognized as an emerging pathogen, with limited information or statistics on this pathogen. Moreover, poor hygienic practices in the unorganized meat sector favor its growth and survival. Therefore, the present study was carried out in Punjab, India to assess the prevalence of Listeria spp. in chevon and mutton samples, followed by its characterization. The prevalence of Listeria in chevon, mutton and swab samples collected from butchers' shops and slaughter houses was 1.82%, 3.21% and 6.66%, respectively. The 18 (2.67%) L. monocytogenes strains isolated from 786 samples carried one or more virulence-associated gene. Based on virulence genes, 6/18 isolates were potentially pathogenic. Serovar 4b (44.4%) was predominant, followed by serovars 1/2a (22.2%), 1/2b (22.2%) and 1/2c (11.1%). Detection of L. monocytogenes in meat and environment samples indicated poor hygiene, potential cross-contamination and risk of listeriosis to consumers and occupational workers. The findings of the study were also relevant to the fact that most of the slaughtering in Punjab, India is carried out in unorganized way, and this meat is not stored propery. Moreover, in the absence of a systematic foodborne disease surveillance and monitoring program, a database on foodborne diseases is absent, and needs to be established.
李斯特菌被认为是一种新兴的病原体，关于这种病原体的信息或统计数据有限。此外，非正规肉类部门卫生条件差，有利于其生长和存活。因此，本研究在印度旁遮普邦进行，以评估沙瓦玛和羊肉样本中李斯特菌属的流行情况，并对其进行特征描述。从肉铺和屠宰场采集的沙瓦玛、羊肉和拭子样本中李斯特菌的流行率分别为 1.82%、3.21%和 6.66%。从 786 个样本中分离出的 18 株（2.67%）单核细胞增生李斯特菌携带一个或多个毒力相关基因。基于毒力基因，有 6/18 株分离株具有潜在致病性。血清型 4b（44.4%）最为常见，其次是血清型 1/2a（22.2%）、1/2b（22.2%）和 1/2c（11.1%）。在肉类和环境样本中检测到李斯特菌表明卫生条件差，存在潜在的交叉污染风险，对消费者和职业工人有李斯特菌病感染风险。本研究的结果也与印度旁遮普邦大多数屠宰是在非正规方式进行，而且这些肉品没有得到妥善储存的事实有关。此外，由于缺乏系统的食源性疾病监测和监测计划，目前还没有食源性疾病数据库，需要建立。
