Department of Chemistry , University of Wisconsin , 1101 University Avenue , Madison , Wisconsin 53706 , United States.
School of Pharmacy , University of Wisconsin , 777 Highland Avenue , Madison , Wisconsin 53705 , United States.
J Proteome Res. 2019 Jul 5;18(7):2759-2770. doi: 10.1021/acs.jproteome.9b00026. Epub 2019 Jun 10.
The decrease of pH level in the water affects animals living in aquatic habitat, such as crustaceans. The molecular mechanisms enabling these animals to survive this environmental stress remain unknown. To understand the modulatory function of neuropeptides in crustaceans when encountering drops in pH level, we developed and implemented a multifaceted mass spectrometric platform to investigate the global neuropeptide changes in response to water acidification in the Atlantic blue crab, Callinectes sapidus. Neural tissues were collected at different incubation periods to monitor dynamic changes of neuropeptides under different stress conditions occurring in the animal. Neuropeptide families were found to exhibit distinct expression patterns in different tissues and even each isoform had its specific response to the stress. Circulating fluid in the crabs (hemolymph) was also analyzed after 2-h exposure to acidification, and together with results from tissue analysis, enabled the discovery of neuropeptides participating in the stress accommodation process as putative hormones. Two novel peptide sequences were detected in the hemolymph that appeared to be involved in the stress-related regulation in the crabs.
水体 pH 值下降会影响水生栖息地中的动物，如甲壳类动物。这些动物如何通过分子机制来适应这种环境压力尚不清楚。为了了解神经肽在甲壳类动物应对 pH 值下降时的调节功能，我们开发并实施了一种多方面的质谱平台，以研究大西洋蓝蟹 Callinectes sapidus 对水酸化的全球神经肽变化。在不同的孵育期收集神经组织，以监测在动物体内发生的不同应激条件下神经肽的动态变化。发现神经肽家族在不同组织中表现出不同的表达模式，甚至每种同工型对压力都有其特定的反应。在暴露于酸化 2 小时后，还分析了螃蟹的循环液（血淋巴），并与组织分析结果相结合，发现了参与应激适应过程的神经肽作为潜在的激素。在血淋巴中检测到两种新的肽序列，它们似乎参与了螃蟹的应激相关调节。
