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核蛋白H2B的下调诱导水杨酸介导的对PVX感染的防御反应。 （注：原文句末“in.”表述不完整，推测可能是in plants之类，这里按完整合理语义翻译）

Downregulation of Nuclear Protein H2B Induces Salicylic Acid Mediated Defense Against PVX Infection in .

作者信息

Yang Xue, Lu Yuwen, Zhao Xing, Jiang Liangliang, Xu Shengchun, Peng Jiejun, Zheng Hongying, Lin Lin, Wu Yuanhua, MacFarlane Stuart, Chen Jianping, Yan Fei

机构信息

Department of Plant Protection, Shenyang Agricultural University, Shenyang, China.

Institute of Plant Virology, Ningbo University, Ningbo, China.

出版信息

Front Microbiol. 2019 May 8;10:1000. doi: 10.3389/fmicb.2019.01000. eCollection 2019.

DOI:10.3389/fmicb.2019.01000
PMID:31134032
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6517552/
Abstract

Histone H2B protein is not only structurally important for chromosomal DNA packaging but is also involved in the regulation of gene expression, including the immune response of plants against pathogens. In this study, we show that the potato virus X (PVX) infection resulted in the reduced expression of H2B at both the mRNA and protein level in . Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) was then used to down-regulate the expression of H2B in and tests showed that the titre of TRV was similar in these plants to that in control treated plants. When these H2B-silenced plants were inoculated with PVX, the virus spread more slowly through the plant and there was a lower titre of PVX compared to non-silenced plants. Abnormal leaf development and stem necrosis were observed in the H2B-silenced plants, which were alleviated in H2B-silenced transgenic plants suggesting the involvement of salicylic acid (SA) in the production of these symptoms. Indeed, quantitative reverse transcription (qRT)-PCR and liquid chromatography tandem mass spectroscopy (LC-MS) results showed that endogenous SA is increased in H2B-silenced . Thus, downregulation of H2B induced the accumulation of endogenous SA, which was correlated with stem necrosis and a decreased accumulation of PVX in .

摘要

组蛋白H2B不仅在染色体DNA包装的结构方面具有重要作用，还参与基因表达的调控，包括植物对病原体的免疫反应。在本研究中，我们发现马铃薯X病毒（PVX）感染导致烟草中H2B在mRNA和蛋白质水平上的表达均降低。随后利用基于烟草脆裂病毒（TRV）的病毒诱导基因沉默（VIGS）下调烟草中H2B的表达，检测表明这些植物中TRV的滴度与对照处理植物中的相似。当用PVX接种这些H2B沉默的植物时，与未沉默的植物相比，病毒在植物中的传播更慢，PVX的滴度更低。在H2B沉默的植物中观察到叶片发育异常和茎坏死，而在H2B沉默的转基因植物中这些症状得到缓解，这表明水杨酸（SA）参与了这些症状的产生。事实上，定量逆转录（qRT）-PCR和液相色谱串联质谱（LC-MS）结果表明，H2B沉默的烟草中内源性SA增加。因此，H2B的下调诱导了内源性SA的积累，这与茎坏死以及烟草中PVX积累的减少相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/7de2eca1c13d/fmicb-10-01000-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/bcd67021805a/fmicb-10-01000-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/9be0dcf11482/fmicb-10-01000-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/f6bd8d03e1d3/fmicb-10-01000-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/59a93c83b131/fmicb-10-01000-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/3353c520c129/fmicb-10-01000-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/7de2eca1c13d/fmicb-10-01000-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/bcd67021805a/fmicb-10-01000-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/9be0dcf11482/fmicb-10-01000-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/f6bd8d03e1d3/fmicb-10-01000-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/59a93c83b131/fmicb-10-01000-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/3353c520c129/fmicb-10-01000-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ff8/6517552/7de2eca1c13d/fmicb-10-01000-g006.jpg

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