Babaev V R, Kliustov V N, Misharin A Iu
Arkh Patol. 1987;49(5):35-40.
Localization of lipoproteins of high, low and very low density in the wall of human aorta was studied by an indirect immunofluorescence technique using antibodies to apoproteins (apo) A-I, B-100 and C-III. Apo A-I and apo C-III were distributed relatively evenly through the thickness of the vessel without visible damage, with some prevalence in the zone of lipid infiltration. Apo B-100 were revealed in significant amount in the intima if their content in the aortic media was low. The content of apo A-I and apo C-III was somewhat increased in the lipid strip. Massive deposits of apo B-100 which were frequently distributed unevenly were found in the fibrous plaque. Pretreatment of nonfixed cryostat sections with saline solution containing bovine serum albumin produced a diffused decrease in the intensity of specific fluorescence this being due to the release of lipoproteins from the tissues. The mechanism of the low density proteins accumulation in the aorta, their binding to the vascular wall components is discussed.
采用针对载脂蛋白（apo）A-I、B-100和C-III的抗体，运用间接免疫荧光技术研究了人主动脉壁中高密度、低密度和极低密度脂蛋白的定位。apo A-I和apo C-III在血管厚度上分布相对均匀，未见明显损伤，在脂质浸润区域有一定优势。如果主动脉中膜中apo B-100的含量较低，则在内膜中可检测到大量该蛋白。脂质条带中apo A-I和apo C-III的含量略有增加。在纤维斑块中发现大量apo B-100沉积物，且分布往往不均匀。用含牛血清白蛋白的盐溶液对未固定的冰冻切片进行预处理，会使特异性荧光强度呈弥漫性降低，这是由于脂蛋白从组织中释放所致。文中讨论了低密度蛋白在主动脉中积累及其与血管壁成分结合的机制。
