Ashtekar M D, Dhalla A S, Mazarello T B, Samuel A M
Clin Immunol Immunopathol. 1987 Oct;45(1):29-34. doi: 10.1016/0090-1229(87)90108-5.
Radioimmunoassay (RIA) techniques have been evaluated to detect specific tubercular antigen (TB Ag) and antitubercular antibody (TB Ab) in CSF and serum of patients with tuberculous meningitis (TBM). A solid-phase RIA using H37RV sonicate antigen of Mycobacterium tuberculosis, anti-BCG antibody, and staphylococcal protein A was standardized. TB Ag and TB Ab levels were noted to be significantly elevated in cerebrospinal fluid (CSF) as well in circulating immune complexes (CIC) isolated from serum samples of TBM patients as compared to control group (P less than 0.01). Detectability of disease by demonstrating elevated TB Ag and/or TB Ab levels in either CSF or CIC or both was 95%. There was no correlation between individual levels of TB Ag and TB Ab in CSF and in circulation. A follow-up study in patient over a period of 4-12 weeks revealed that TB antigen and/or TB Ab persisted in the majority of the cases for several weeks despite chemotherapy.
已对放射免疫测定（RIA）技术进行评估，以检测结核性脑膜炎（TBM）患者脑脊液和血清中的特异性结核抗原（TB Ag）和抗结核抗体（TB Ab）。使用结核分枝杆菌H37RV超声破碎抗原、抗卡介苗抗体和葡萄球菌蛋白A的固相RIA已标准化。与对照组相比，TBM患者脑脊液（CSF）以及从血清样本中分离出的循环免疫复合物（CIC）中的TB Ag和TB Ab水平显著升高（P小于0.01）。通过证明脑脊液或CIC或两者中TB Ag和/或TB Ab水平升高来检测疾病的可检测率为95%。脑脊液和循环中TB Ag和TB Ab的个体水平之间没有相关性。对患者进行的为期4至12周的随访研究表明，尽管进行了化疗，但大多数病例中的TB抗原和/或TB Ab仍持续数周。
