柳叶菜科（Onagraceae）简单序列重复标记的开发。
Development of simple sequence repeat markers for (Onagraceae).
作者信息
Mi Rui, Wang Tongcheng, Dunn Derek W, Huang Kang, Li Baoguo
机构信息
Shaanxi Key Laboratory for Animal Conservation College of Life Sciences Northwest University Xi'an 710069 People's Republic of China.
Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences Kunming 650223 People's Republic of China.
出版信息
Appl Plant Sci. 2019 May 8;7(5):e01244. doi: 10.1002/aps3.1244. eCollection 2019 May.
PREMISE
Rosebay willowherb, or fireweed (: Onagraceae), has diploid, tetraploid, and hexaploid cytotypes. There are known physiological and ecological differences among the three cytotypes, but genetic differences remain undetermined. We developed simple sequence repeat (SSR) markers for this species.
METHODS AND RESULTS
Leaf samples were collected from three hexaploid populations. We successfully amplified 16 SSR loci, which were found to be highly polymorphic. The number of alleles, the observed heterozygosity levels, and the expected heterozygosity levels ranged from four to 13, 0.286-0.899, and 0.372-0.871, respectively. Most primers could also be amplified successfully in and the closely related species .
CONCLUSIONS
The 16 polymorphic SSR markers developed here will be useful for genetic studies in and related species.
前提
柳叶菜，即柳叶菜属（柳叶菜科），具有二倍体、四倍体和六倍体细胞型。已知这三种细胞型之间存在生理和生态差异，但遗传差异仍未确定。我们为该物种开发了简单序列重复（SSR）标记。
方法与结果
从三个六倍体种群中采集叶片样本。我们成功扩增出16个SSR位点，发现它们具有高度多态性。等位基因数量、观察到的杂合度水平和预期杂合度水平分别为4至13、0.286 - 0.899和0.372 - 0.871。大多数引物也能在[此处原文可能有遗漏相关物种信息]及其近缘物种中成功扩增。
结论
这里开发的16个多态性SSR标记将有助于柳叶菜及其相关物种的遗传研究。
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