分枝杆菌的代谢研究。IV. 麻风分枝杆菌中异柠檬酸裂解酶和苹果酸合酶活性的测定。

Metabolic studies on mycobacteria. IV. Assay of isocitrate lyase and malate synthase activity in M. leprae.

作者信息

Bharadwaj V P, Katoch V M, Sharma V D, Kannan K B, Datta A K, Shivannavar C T

机构信息

Division of Laboratory Research, Central JALMA Institute for Leprosy, (I.C.M.R.), Taj Ganj.

出版信息

Indian J Lepr. 1987 Apr-Jun;59(2):158-62.

PMID:3116105

Abstract

Cell free extracts from M. tuberculosis H37 Rv, M. smegmatis armadillo derived M. leprae and normal armadillo liver homogenates were assayed for the presence of isocitrate lyase and malate synthase activity. It was observed that significant amount of isocitrate lyase and malate synthase activity was present in M. tuberculosis H37 Rv, M. smegmatis and armadillo derived M. leprae. No such activity was demonstrable in cell free extracts of normal armadillo liver. It is concluded that M. leprae like other mycobacteria has the capability to metabolise via glyoxylate bypass of TCA cycle. These findings may be relevant for understanding the energy metabolism of M. leprae under stress conditions and possibly the 'persister' stage.

摘要

对结核分枝杆菌H37 Rv、犰狳来源的耻垢分枝杆菌和正常犰狳肝脏匀浆的无细胞提取物进行了异柠檬酸裂解酶和苹果酸合酶活性检测。结果发现，结核分枝杆菌H37 Rv、耻垢分枝杆菌和犰狳来源的麻风分枝杆菌中存在大量异柠檬酸裂解酶和苹果酸合酶活性。在正常犰狳肝脏的无细胞提取物中未检测到此类活性。结论是，与其他分枝杆菌一样，麻风分枝杆菌有能力通过三羧酸循环的乙醛酸旁路进行代谢。这些发现可能有助于理解麻风分枝杆菌在应激条件下以及可能在“持留菌”阶段的能量代谢。