发展和优化 pH 响应性 PLGA-壳聚糖纳米粒用于触发释放抗菌药物。
Development and optimization of pH-responsive PLGA-chitosan nanoparticles for triggered release of antimicrobials.
机构信息
Department of Food Technology, Federal University of Viçosa, Viçosa, MG 36570-900, Brazil; Department of Biological & Agricultural Engineering, Texas A&M University, College Station, TX 77843-2117, United States.
Agricultural Science Institute, Federal University of Viçosa, Rio Paranaíba, MG 38810-000, Brazil.
出版信息
Food Chem. 2019 Oct 15;295:671-679. doi: 10.1016/j.foodchem.2019.05.165. Epub 2019 May 24.
The aim of this work was to develop and optimize a pH-responsive nanoparticle based on poly(D,L-lactide-co-glycolide) (PLGA) and chitosan (CHIT) for delivery of natural antimicrobial using trans-cinnamaldehyde (TCIN) as a model compound. The optimization was performed using a central composite design and the desirability function approach. The optimized levels of variables considering all significant responses were 4% (w/w) of TCIN and 6.75% (w/w) of CHIT. After, optimized nanoparticles were produced and characterized according to their physicochemical properties and their antimicrobial activity against Salmonella Typhimurium and Staphylococcus aureus. Optimized nanoparticles characterization indicated a satisfactory TCIN encapsulation (33.20 ± 0.85%), spherical shape, pH-responsive controlled release, with faster release in the presence of CHIT at low pH, and enhanced antimicrobial activity against both pathogens. TCIN encapsulation using PLGA coated with CHIT enhanced its antimicrobial activity and generated a delivery system with pH-sensitivity for controlled release with promising properties for food safety applications.
本工作旨在开发和优化一种基于聚（D，L-丙交酯-共-乙交酯）（PLGA）和壳聚糖（CHIT）的 pH 响应性纳米粒子，以使用反式肉桂醛（TCIN）作为模型化合物来递送天然抗菌剂。使用中心组合设计和理想函数方法进行了优化。考虑到所有显著响应，优化变量的水平为 4%（w/w）的 TCIN 和 6.75%（w/w）的 CHIT。然后，根据其物理化学性质和对肠炎沙门氏菌和金黄色葡萄球菌的抗菌活性来生产和表征优化后的纳米粒子。优化后的纳米粒子的特性表明，TCIN 的包封率（33.20±0.85%）令人满意，呈球形，具有 pH 响应性的控制释放特性，在低 pH 下存在 CHIT 时释放速度更快，对两种病原体的抗菌活性增强。使用 CHIT 涂覆的 PLGA 包封 TCIN 增强了其抗菌活性，并生成了一种具有 pH 敏感性的控释递送系统，具有用于食品安全应用的有前景的特性。
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