真空采血管中的残余负压与儿科血液标本溶血
Residual Negative Pressure in Vacuum Blood-Collection Tube and Hemolysis in Pediatric Blood Specimens.
作者信息
Hu Jing, Zhang Qiao-Xin, Xiao Tong-Tong, Pan Mei-Chen, Cai Ying-Mu
机构信息
Department of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, China.
出版信息
Lab Med. 2020 Jan 2;51(1):41-46. doi: 10.1093/labmed/lmz026.
OBJECTIVE
To determine a method to reduce specimen hemolysis rates in pediatric blood specimens.
METHODS
A total of 290 blood specimens from pediatric patients were classified into the capped group or uncapped group. The hemolysis index and levels of lactate dehydrogenase (LDH) were measured using an automated biochemical analyzer. Also, we performed a paired test to measure the concentration of free hemoglobin in specimens from 25 randomly selected healthy adult volunteers, using a direct spectrophotometric technique.
RESULTS
The hemolytic rate of capped specimens was 2-fold higher than that of uncapped specimens. We found significant differences for LDH. Also, there was a significant difference in the concentration of free hemoglobin in the random-volunteers test.
CONCLUSIONS
Eliminating the residual negative pressure of vacuum blood-collection tubes was effective at reducing the macrohemolysis and/or microhemolysis rate.
目的
确定一种降低儿科血液标本溶血率的方法。
方法
将290份儿科患者的血液标本分为加盖组和未加盖组。使用自动生化分析仪测量溶血指数和乳酸脱氢酶（LDH）水平。此外，我们采用直接分光光度法对25名随机挑选的健康成年志愿者的标本进行配对检测，以测量游离血红蛋白浓度。
结果
加盖标本的溶血率比未加盖标本高2倍。我们发现LDH存在显著差异。在随机志愿者检测中，游离血红蛋白浓度也存在显著差异。
结论
消除真空采血管的残余负压可有效降低大溶血和/或微溶血率。
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