Tiwari Jagesh Kumar, Ali Nilofer, Devi Sapna, Zinta Rasna, Kumar Vinod, Chakrabarti Swarup Kumar
ICAR-Central Potato Research Institute, Shimla, Himachal Pradesh 171001 India.
3 Biotech. 2019 Jul;9(7):262. doi: 10.1007/s13205-019-1785-6. Epub 2019 Jun 11.
Allelic variation in wild potato () species was analysed using 14 simple sequence repeat (SSR) markers. SSR allelic profiles showed high polymorphism and distinctness among the wild species. A total of 109 alleles of 14 polymorphic SSR markers were scored in 82 accessions belonging to 22 wild potato species. Allele size ranged from a minimum of 104 bp (STI0030) to a maximum of 304 bp (STM5114). Number of SSR alleles per marker ranged from 4 (STM5127/STM1053) to 13 (STM0019), whereas PIC value varied between 0.66 (STM1053) and 0.91 (STM0019). Cluster analysis using SSR allelic profiles of 82 accessions grouped showed 5 major clusters (I-V) based on the Dice similarity coefficient using neighbour-joining clustering method. Distinct allelic variations were observed among the accessions irrespective of the origin country, series and species. Our study suggests that SSR-based molecular characterization of wild potato species is accession specific and development of an allelic dataset for all the accessions would strengthen their utilization in potato research in future.
利用14个简单序列重复（SSR）标记对野生马铃薯（）物种的等位基因变异进行了分析。SSR等位基因谱显示野生种间具有高度多态性和独特性。在属于22个野生马铃薯物种的82份材料中，对14个多态性SSR标记的109个等位基因进行了评分。等位基因大小范围从最小的104 bp（STI0030）到最大的304 bp（STM5114）。每个标记的SSR等位基因数从4个（STM5127/STM1053）到13个（STM0019）不等，而PIC值在0.66（STM1053）和0.91（STM0019）之间变化。使用邻接法聚类方法，基于Dice相似系数，对82份材料的SSR等位基因谱进行聚类分析，结果显示有5个主要聚类（I-V）。无论材料的原产国、系列和物种如何，在材料间均观察到明显的等位基因变异。我们的研究表明，野生马铃薯物种基于SSR的分子特征是特定于材料的，为所有材料开发等位基因数据集将加强其在未来马铃薯研究中的利用。
