不同动物物种中特定α-1,2-葡萄糖苷酶对葡萄糖基半乳糖基羟赖氨酸活性的比较研究。

Comparative study of specific alpha-1,2-glucosidase activity toward glucosyl galactosyl hydroxylysine in various animal species.

Ishii I, Iwase H, Hamazaki H, Hotta K

Department of Biochemistry, School of Medicine, Kitasato University, Kanagawa, Japan.

Comp Biochem Physiol B. 1987;88(1):313-6. doi: 10.1016/0305-0491(87)90121-0.

Abstract

Assay of the activity of alpha-1,2-glucosidase was completed within 10 min using reversed-phase high performance liquid chromatography and purified dansylated glucosyl galactosyl hydroxylysine as the substrate. 2. A comparative study was made on the enzyme activity of liver homogenate from eight animal species, mouse, frog, chicken, rabbit, pig, rat, human, bovine and that of a spinach leaf homogenate. alpha-1,2-glucosidase activity in human and bovine liver was very low, and that of alpha-1,2-glucosidase could not be detected in the spinach homogenate as expected. 3. 1-deoxynojirimycin, a well known potent inhibitor of alpha-1,2-glucosidases which act on the N-glycosidic type carbohydrate chain, also inhibited alpha-1,2-glucosidase acting specificity on glucosyl galactosyl hydroxylysine derived from the collagen molecule.

摘要

使用反相高效液相色谱法，并以纯化的丹磺酰化葡萄糖基半乳糖基羟赖氨酸为底物，在10分钟内完成了α-1,2-葡萄糖苷酶活性的测定。2. 对来自八种动物物种（小鼠、青蛙、鸡、兔子、猪、大鼠、人类、牛）的肝脏匀浆以及菠菜叶匀浆的酶活性进行了比较研究。人类和牛肝脏中的α-1,2-葡萄糖苷酶活性非常低，并且正如预期的那样，在菠菜匀浆中未检测到α-1,2-葡萄糖苷酶的活性。3. 1-脱氧野尻霉素是一种作用于N-糖苷型碳水化合物链的著名强效α-1,2-葡萄糖苷酶抑制剂，它也抑制了α-1,2-葡萄糖苷酶对源自胶原蛋白分子的葡萄糖基半乳糖基羟赖氨酸的作用特异性。