利用基因鉴定中华鳖（鳖科）的性别。

Identification of sex using gene in the Chinese softshell turtle, (Trionychidae).

作者信息

Zhao Lan, Wang Xin, Wan Qiu-Hong, Fang Sheng-Guo

机构信息

The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Centre for Gene Resources of Endangered Wildlife, College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.

出版信息

J Genet. 2019 Mar;98(1).

PMID:31204713

Abstract

The Chinese softshell turtle exhibits ZZ/ZW sex determination. To identify the sex of embryos, juvenile and adult individuals, we designed two pairs of polymerase chain reaction primers, SB1-196, which amplifies a fragment of 196 bp in the female and the other, CK1-482, which amplifies the 482-bp fragment in both the sexes. It is validated in 24 adult turtles of known sex, sampled from three different locations. This one-step sexing technique is rapid and easy to perform and is reported for the first time.

摘要

中华鳖表现出ZZ/ZW性别决定方式。为了鉴定胚胎、幼年和成年个体的性别，我们设计了两对聚合酶链反应引物，SB1-196，它在雌性个体中扩增出一个196 bp的片段，另一对引物CK1-482，它在两性个体中都能扩增出482 bp的片段。该方法在从三个不同地点采集的24只已知性别的成年鳖中得到了验证。这种一步法性别鉴定技术快速且易于操作，是首次报道。